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          Institute: MPI für molekulare Physiologie     Collection: Abteilung II - Systemische Zellbiologie     Display Documents



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ID: 427503.0, MPI für molekulare Physiologie / Abteilung II - Systemische Zellbiologie
Dynamics and segregation of cell-matrix adhesions in cultured fibroblasts
Authors:Zamir, E.; Katz, M.; Posen, Y.; Erez, N.; Yamada, K. M.; Katz, B. Z.; Lin, S.; Lin, D. C.; Bershadsky, A.; Kam, Z.; Geiger, B.
Date of Publication (YYYY-MM-DD):2000-04
Title of Journal:Nature Cell Biology
Journal Abbrev.:Nat Cell Biol
Volume:2
Issue / Number:4
Start Page:191
End Page:196
Review Status:not specified
Audience:Not Specified
Abstract / Description:Here we use time-lapse microscopy to analyse cell-matrix adhesions in cells expressing one of two different cytoskeletal proteins, paxillin or tensin, tagged with green fluorescent protein (GFP). Use of GFP-paxillin to analyse focal contacts and GFP-tensin to study fibrillar adhesions reveals that both types of major adhesion are highly dynamic. Small focal contacts often translocate, by extending centripetally and contracting peripherally, at a mean rate of 19 micrometers per hour. Fibrillar adhesions arise from the medial ends of stationary focal contacts, contain alpha5beta1 integrin and tensin but not other focal-contact components, and associate with fibronectin fibrils. Fibrillar adhesions translocate centripetally at a mean rate of 18 micrometers per hour in an actomyosin-dependent manner. We propose a dynamic model for the regulation of cell-matrix adhesions and for transitions between focal contacts and fibrillar adhesions, with the ability of the matrix to deform functioning as a mechanical switch.
Free Keywords:Actins/metabolism; Actomyosin/metabolism; Bicyclo Compounds, Heterocyclic/pharmacology; Cell Adhesion/*physiology; Cells, Cultured; Cytoskeletal Proteins/genetics/*metabolism; Cytoskeleton/drug effects/*physiology; Extracellular Matrix/*physiology; Fibroblasts/cytology; Genes, Reporter; Green Fluorescent Proteins; Humans; Indicators and Reagents/metabolism; Luminescent Proteins/genetics; Microfilament Proteins/genetics/*metabolism; Paxillin; Phosphoproteins/genetics/*metabolism; Recombinant Proteins/genetics/metabolism; Thiazoles/pharmacology; Thiazolidines; Transfection
External Publication Status:published
Document Type:Article
Communicated by:Jürgen Block
Affiliations:MPI für molekulare Physiologie/Abteilung II - Systemische Zellbiologie/AG Dr. E. Zamir
External Affiliations:%G eng
Identifiers:ISSN:1465-7392 (Print) %R 10.1038/35008607 [ID No:1]
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=... [ID No:2]
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