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          Institute: MPI für Herz- und Lungenforschung (W. G. Kerckhoff Institut)     Collection: Publikationen des W. G. Kerckhoff-Instituts     Display Documents

ID: 428415.0, MPI für Herz- und Lungenforschung (W. G. Kerckhoff Institut) / Publikationen des W. G. Kerckhoff-Instituts
Characterization of a murine model of monocrotaline pyrrole-induced acute lung injury
Authors:Dumitrascu, R.; Koebrich, S.; Dony, E.; Weissmann, N.; Savai, R.; Pullamsetti, S. S.; Ghofrani, H. A.; Samidurai, A.; Traupe, H.; Seeger, W.; Grimminger, F.; Schermuly, R. T.
Date of Publication (YYYY-MM-DD):2008
Title of Journal:BMC Pulm Med
Issue / Number:1
Start Page:25
Audience:Not Specified
Abstract / Description:ABSTRACT: Backgroud: New animal models of chronic pulmonary hypertension in mice are needed. The injection of monocrotaline is an established model of pulmonary hypertension in rats. The aim of this study was to establish a murine model of pulmonary hypertension by injection of the active metabolite, monocrotaline pyrrole. METHODS: Survival studies, computed tomographic scanning, histology, bronchoalveolar lavage were performed, and arterial blood gases and hemodynamics were measured in animals which received an intravenous injection of different doses of monocrotaline pyrrole. RESULTS: Monocrotaline pyrrole induced pulmonary hypertension in Sprague Dawley rats. When injected into mice, monocrotaline pyrrole induced dose-dependant mortality in C57Bl6/N and BALB/c mice (dose range 6-15 mg/kg bodyweight). At a dose of 10 mg/kg bodyweight, mice developed a typical early-phase acute lung injury, characterized by lung edema, neutrophil influx, hypoxemia and reduced lung compliance. In the late phase, monocrotaline pyrrole injection resulted in limited lung fibrosis and no obvious pulmonary hypertension. CONCLUSIONS: Monocrotaline and monocrotaline pyrrole pneumotoxicity substantially differs between the animal species.
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:MPI für physiologische und klinische Forschung
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