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Home Search Quick Search Advanced Fulltext Browse Collections Persons My eDoc Session History Login Name: Password: Documentation Help Support Wiki Direct access to document ID:	Institute: MPI für Polymerforschung     Collection: MPI Polymerforschung     Display Documents   history ID: 42969.0, MPI für Polymerforschung / MPI Polymerforschung Kinetics of valinomycin-mediated K+ ion transport through tethered bilayer lipid membranes Authors: Naumann, Renate; Walz, D.; Schiller, S. M.; Knoll, Wolfgang Language: English Date of Publication (YYYY-MM-DD): 2003 Title of Journal: Journal of Electroanalytical Chemistry Journal Abbrev.: J. Electroanal. Chem. Volume: 550-551 Start Page: 241 End Page: 252 Review Status: not specified Audience: Not Specified Abstract / Description: Bilayer lipid membranes tethered to planar gold electrodes were prepared, based on a self assembled monolayer (SAM) of 2,3-di-O-phytanyl-sn-glycerol-1-tetraethylene glycol-DL-α-lipoic acid ester lipid (DPTL). When the SAM's were exposed to a suspension of liposomes made from diphytanoylphosphodatidyl choline (DPhyPC), tethered lipid bilayers (tBLMs) were formed with good sealing properties. The preformed tBLMs were doped with valinomycin, and the K+ ion concentration in the bathing solution was increased stepwise by adding KCl. Electrical impedance spectra were recorded following every addition. These data were modelled by means of the network simulation program SPICE, using parameter values of the undoped membrane and a kinetic scheme for the K+/valinomycin system, whose rate constants were determined previously in independent measurements. Experimental and simulated data are in reasonable agreement despite some simplifying assumptions used in the SPICE simulations. Experimental data were also fitted to a conventional equivalent circuit, which reveals that the representation of the K+/valinomycin system by an ohmic resistor can be considered as no more than an approximation. © 2003 Elsevier Science B.V. All rights reserved. External Publication Status: published Document Type: Article Affiliations: MPI für Polymerforschung External Affiliations: Univ Basel, Biozentrum, Basel, Switzerland Identifiers: LOCALID:P-03-51 The scope and number of records on eDoc is subject to the collection policies defined by each institute - see "info" button in the collection browse view.