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          Institute: MPI für Neurobiologie     Collection: Cellular and Systems Neurobiology     Display Documents

ID: 429926.0, MPI für Neurobiologie / Cellular and Systems Neurobiology
NKCC1-Dependent GABAergic Excitation Drives Synaptic Network Maturation during Early Hippocampal Development
Authors:Pfeffer, C. K.; Stein, V.; Keating, D. J.; Maier, H.; Rinke, I.; Rudhard, Y.; Hentschke, M.; Rune, G. M.; Jentsch, T. J.; Hübner, C. A.
Date of Publication (YYYY-MM-DD):2009-03-18
Title of Journal:Journal of Neuroscience
Journal Abbrev.:J. Neurosci.
Issue / Number:11
Start Page:3419
End Page:3430
Review Status:Peer-review
Audience:Not Specified
Abstract / Description:A high intracellular chloride concentration in immature neurons leads to a depolarizing action of GABA that is thought to shape the developing neuronal network. We show that GABA-triggered depolarization and Ca2+ transients were attenuated in mice deficient for the Na-K-2Cl cotransporter NKCC1. Correlated Ca2+ transients and giant depolarizing potentials (GDPs) were drastically reduced and the maturation of the glutamatergic and GABAergic transmission in CA1 delayed. Brain morphology, synaptic density, and expression levels of certain developmental marker genes were unchanged. The expression of lynx1, a protein known to dampen network activity, was decreased. In mice deficient for the neuronal Cl-/HCO3- exchanger AE3, GDPs were also diminished. These data show that NKCC1-mediated Cl- accumulation contributes to GABAergic excitation and network activity during early postnatal development and thus facilitates the maturation of excitatory and inhibitory synapses.
External Publication Status:published
Document Type:Article
Version Comment:Automatic journal name synchronization
Affiliations:MPI für Neurobiologie/Cellular and Systems Neurobiology (Bonhoeffer)/Synaptic Receptor Trafficking (Stein)
External Affiliations:[Pfeffer, Carsten K.; Jentsch, Thomas J.] Max Delbruck Ctr Mol Med, D-13125 Berlin, Germany.; [Pfeffer, Carsten K.; Jentsch, Thomas J.] Leibniz Inst Mol Pharmakol FMP, D-13125 Berlin, Germany.; [Pfeffer, Carsten K.; Keating, Damien J.; Rudhard, York; Jentsch, Thomas J.; Huebner, Christian A.] Univ Hamburg, ZMNH, D-20251 Hamburg, Germany.; [Maier, Hannes] Univ Klinikum Hamburg Eppendorf, Klin Hals Nasen & Ohrenheilkunde, D-22529 Hamburg, Germany.; [Hentschke, Moritz; Huebner, Christian A.] Univ Klinikum Hamburg Eppendorf, Inst Humangenet, D-22529 Hamburg, Germany.; [Rune, Gabriele M.] Univ Klinikum Hamburg Eppendorf, Inst Anat, D-22529 Hamburg, Germany.; [Huebner, Christian A.] Univ Jena, Inst Klin Chem, D-07747 Jena, Germany.
Identifiers:ISI:000264342100010 [ID No:1]
ISSN:0270-6474 [ID No:2]
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