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          Institute: MPI für Evolutionsbiologie     Collection: Ecophysiology     Display Documents

ID: 430632.0, MPI für Evolutionsbiologie / Ecophysiology
Effect of common bean (Phaseolus vulgaris L.) on the community composition of ammonia-oxidizing bacteria in soil previously cultivated with Medicago sativa
Authors:Junier, Pilar; Carú, Margarita; Witzel, Karl-Paul
Date of Publication (YYYY-MM-DD):2009-06
Title of Journal:European Journal of Soil Biology
Issue / Number:3
Start Page:252
End Page:258
Review Status:not specified
Audience:Not Specified
Abstract / Description:The community composition of ammonia-oxidizing bacteria (AOB) was studied during four stages of plant development in soil cultivated with Phaseolus vulgaris in comparison to unplanted soil, using an alfisol previously harboring the legume Medicago sativa. Denaturing gradient gel electrophoresis (DGGE) patterns of 16S rRNA gene and clone libraries of the same gene suggested that bacteria related to Nitrosospira cluster 3 were dominant in both planted and unplanted soil. Bacteria related to Nitrosomonas cluster 8 (Nitrosomonas communis cluster) were found at all times in planted soil, but appeared only randomly in unplanted soil. Analysis of PCR products of the gene encoding the alpha-subunit of ammonia monooxygenase (amoA) by DGGE and clone libraries only detected Nitrosospira cluster 3-like organisms, but failed to detect sequences related to Nitrosomonas. The results suggest that P. vulgaris does not affect the dominant members of AOB communities (Nitrosospira cluster 3), but could have an effect on the prevalence of Nitrosomonas cluster 8 in this type of legume-planted alfisol.
Free Keywords:Ammonia-oxidizing bacteria; Legumes rhizosphere; Nitrosospira cluster 3; Nitrosomonas cluster 8; amoA; 16S rRNA
External Publication Status:published
Document Type:Article
Affiliations:MPI für Evolutionsbiologie/Abt. Ökophysiologie
External Affiliations:Ecole Polytechnique Federale de Lausanne, CH-1015 Lausanne, Switzerland; Departamento de Ciencias Ecológicas, Facultad de Ciencias, Universidad de Chile, Casilla, 653 Santiago, Chile
Identifiers:ISSN:1164-5563 (print) [ID-No:1]
ISSN:1778-3615 (online) [ID-No:2]
DOI:10.1016/j.ejsobi.2009.01.003 [ID-No:3]
LOCALID:2701/S 39002 [Listen-Nummer/S-Nummer]
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