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          Institute: MPI für Neurobiologie     Collection: Neuroimmunology     Display Documents



  history
ID: 432416.0, MPI für Neurobiologie / Neuroimmunology
The Presence of Lytic HSV-1 Transcripts and Clonally Expanded T Cells with a Memory Effector Phenotype in Human Sensory Ganglia
Authors:Derfuss, T.; Arbusow, V.; Strupp, M.; Brandt, T.; Theil, D.
Language:English
Place of Publication:OXFORD
Publisher:BLACKWELL PUBLISHING
Date of Publication (YYYY-MM-DD):2009
Title of Book:BASIC AND CLINICAL ASPECTS OF VERTIGO AND DIZZINESS
Start Page:300
End Page:304
Full Name of Book-Editor(s):Strupp, Michael et al.
Title of Series:Annals of the New York Academy of Sciences
Volume:1164
Review Status:not specified
Audience:Not Specified
Abstract / Description:Herpes simplex virus type 1 (HSV-1) latent persistence in human trigeminal ganglia (TG) is accompanied by a chronic CD8 T-cell infiltration. Thus far, during HSV-1 latency only a single transcript, namely the latency-associated transcript (LAT), has been identified to be synthesized but not translated into a protein. In contrast, the chronic CD8 T-cell infiltration suggests that an antigen trigger must be present. The focus of the current work was to look for HSV-1 transcription activity as a potential trigger of the immune response and to demonstrate whether the immune cells are clonally expanded and have a phenotype that suggests that they have been triggered by viral antigen. By combining in situ hybridization, laser cutting microscopy, and single-cell real time RT-PCR, we demonstrated expression of the HSV-1 immediate early (IE) genes ICP0 and ICP4 in human trigeminal neurons. Using CDR3 spectratyping, we showed that the infiltrating T cells are clonally expanded, indicating an antigen-driven immune response. Moreover, the persisting CD8(+) T cells had prominent features of the memory effector phenotype. Chemokines CCL5 and CXCL10 were expressed by a subpopulation of infiltrating cells and the corresponding chemokine receptors CCR5 and CXCR3 were co-expressed on virtually all T cells bearing the CD8 phenotype. Thus, HSV-1 IE genes are expressed in human TG, and the infiltrating T cells bear several characteristics that suggest viral antigenic stimulation.
Free Keywords:sensory ganglia; HSV-1; T cells; lytic transcripts; clonal expansion; cranial nerve disorders
External Publication Status:published
Document Type:InBook
Affiliations:MPI für Neurobiologie/Neuroimmunology (Wekerle)/Clinical Neuroimmunology (Hohlfeld)
External Affiliations:[Arbusow, Viktor; Strupp, Michael; Brandt, Thomas; Theil, Diethilde] Univ Munich, Klinikum Grosshadern, Dept Neurol, D-81377 Munich, Germany.; [Derfuss, Tobias] Univ Munich, Inst Clin Neuroimmunol, D-81377 Munich, Germany.
Identifiers:ISI:000267019700042 [ID No:1]
ISSN:0077-8923 [ID No:2]
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