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          Institute: MPI für Neurobiologie     Collection: Cellular and Systems Neurobiology     Display Documents



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ID: 435262.0, MPI für Neurobiologie / Cellular and Systems Neurobiology
Long-term, high-resolution imaging in the mouse neocortex through a chronic cranial window
Authors:Holtmaat, A.; Bonhoeffer, T.; Chow, D. K.; Chuckowree, J.; De Paola, V.; Hofer, S. B.; Huebener, M.; Keck, T.; Knott, G.; Lee, W. C. A.; Mostany, R.; Mrsic-Flogel, T. D.; Nedivi, E.; Portera-Cailliau, C.; Svoboda, K.; Trachtenberg, J. T.; Wilbrecht, L.
Language:English
Date of Publication (YYYY-MM-DD):2009
Title of Journal:Nature Protocols
Journal Abbrev.:Nat. Protoc.
Volume:4
Issue / Number:8
Start Page:1128
End Page:1144
Review Status:Peer-review
Audience:Not Specified
Abstract / Description:To understand the cellular and circuit mechanisms of experience-dependent plasticity, neurons and their synapses need to be studied in the intact brain over extended periods of time. Two-photon excitation laser scanning microscopy (2PLSM), together with expression of fluorescent proteins, enables high-resolution imaging of neuronal structure in vivo. In this protocol we describe a chronic cranial window to obtain optical access to the mouse cerebral cortex for long-term imaging. A small bone flap is replaced with a coverglass, which is permanently sealed in place with dental acrylic, providing a clear imaging window with a large field of view (similar to 0.8-12 mm(2)). The surgical procedure can be completed within similar to 1 h. The preparation allows imaging over time periods of months with arbitrary imaging intervals. The large size of the imaging window facilitates imaging of ongoing structural plasticity of small neuronal structures in mice, with low densities of labeled neurons. The entire dendritic and axonal arbor of individual neurons can be reconstructed.
External Publication Status:published
Document Type:Article
Version Comment:Automatic journal name synchronization
Affiliations:MPI für Neurobiologie/Cellular and Systems Neurobiology (Bonhoeffer)
External Affiliations:[Chow, David K.; Trachtenberg, Joshua T.] Univ Calif Los Angeles, Dept Neurobiol, Los Angeles, CA USA.; [Chuckowree, Jyoti; Knott, Graham] Univ Lausanne, Dept Cell Biol & Morphol, Lausanne, Switzerland.; [Lee, Wei-Chung A.; Nedivi, Elly] MIT, Dept Brain & Cognit Sci & Biol, Picower Inst Learning & Memory, Cambridge, MA USA.; [Mostany, Ricardo; Portera-Cailliau, Carlos] Univ Calif Los Angeles, Reed Neurol Res Ctr, Dept Neurol, Los Angeles, CA 90024 USA.; [Mostany, Ricardo; Portera-Cailliau, Carlos] Univ Calif Los Angeles, Reed Neurol Res Ctr, Dept Neurobiol, Los Angeles, CA 90024 USA.; [Holtmaat, Anthony; De Paola, Vincenzo; Svoboda, Karel; Wilbrecht, Linda] Howard Hughes Med Inst, Cold Spring Harbor Lab, New York, NY USA.
Identifiers:ISI:000268858800004 [ID No:1]
ISSN:1754-2189 [ID No:2]
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