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          Institute: MPI für Infektionsbiologie     Collection: Department of Immunology     Display Documents



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ID: 441546.0, MPI für Infektionsbiologie / Department of Immunology
Expression of catalytic proteasome subunits in the gut of patients with Crohn's disease
Authors:Visekruna, Alexander; Slavova, Nadia; Dullat, Sonja; Gröne, Jörn; Kroesen, Anton-Josef; Ritz, Jörg-Peter; Buhr, Heinz-Johannes; Steinhoff, Ulrich
Language:English
Date of Publication (YYYY-MM-DD):2009-10
Title of Journal:International Journal of Colorectal Disease
Volume:24
Issue / Number:10
Start Page:1133
End Page:1139
Copyright:© The Author(s) 2009
This article is published with open access at Springerlink.com
Open Access This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
Review Status:not specified
Audience:Experts Only
Abstract / Description:Background and purpose Activation of the transcription factor NF-kappa B by proteasomes and subsequent nuclear translocation of cytoplasmatic complexes play a crucial role in the intestinal inflammation. Proteasomes have a pivotal function in NF-kappa B activation by mediating degradation of inhibitory I kappa B proteins and processing of NF-kappa B precursor proteins. This study aims to analyze the expression of the human proteasome subunits in colonic tissue of patients with Crohn's disease. Materials and methods Thirteen patients with Crohn's disease and 12 control patients were studied. The expression of immunoproteasomes and constitutive proteasomes was examined by Western blot analysis, immunoflourescence and quantitative real-time PCR. For real-time PCR, AK2C was used as housekeeping gene. Results The results indicate the influence of the intestinal inflammation on the expression of the proteasomes in Crohn's disease. Proteasomes from inflamed intestine of patients with Crohn's disease showed significantly increased expression of immunosubunits on both protein and mRNA levels. Especially, the replacement of the constitutive proteasome subunit beta 1 by inducible immunosubunit beta 1i was observed in patients with active Crohn's disease. In contrast, relatively low abundance of immunoproteasomes was found in control tissue. Conclusions Our data demonstrate that in contrast to normal colonic tissue, the expression of immunoproteasomes was evidently increased in the inflamed colonic mucosa of patients with Crohn's disease. Thus, the chronic intestinal inflammation process in Crohn's disease leads to significant alterations of proteasome subsets.
Free Keywords:Crohn’s disease - Inflammatory bowel disease - 26S proteasome - 20S proteasome - NF-κB
Comment of the Author/Creator:Acknowledgements
This work was supported by Sonderforschungsbereich 633 (Berlin, Germany).
External Publication Status:published
Document Type:Article
Communicated by:Hilmar Fünning
Affiliations:MPI für Infektionsbiologie/Department of Immunology
External Affiliations:Department of Surgery I, Charité-Medical School, Campus Benjamin Franklin, Hindenburgdamm 30, 12200 Berlin, Germany
Identifiers:ISI:000269376800002 [ID No:1]
ISSN:0179-1958 [ID No:2]
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