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| ID:
444325.0,
MPI für Infektionsbiologie / Department of Immunology |
| Identification of T-cell antigens specific for latent mycobacterium tuberculosis infection. |
| Authors: | Schuck, Sebastian D.; Mueller, Henrik; Kunitz, Frank; Neher, Albert; Hoffmann, Harald; Franken, Kees L. C. M.; Repsilber, Dirk; Ottenhoff, Tom H. M.; Kaufmann, Stefan H. E.; Jacobsen, Marc | | Language: | English | | Date of Publication (YYYY-MM-DD): | 2009-05 | | Title of Journal: | PLoS ONE | | Volume: | 4 | | Issue / Number: | 5 | | Sequence Number of Article: | e5590 | | Copyright: | © 2009 Schuck et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | | Review Status: | not specified | | Audience: | Experts Only | | Abstract / Description: | BACKGROUND: T-cell responses against dormancy-, resuscitation-, and reactivation-associated antigens of Mycobacterium tuberculosis are candidate biomarkers of latent infection in humans. METHODOLOGY/PRINCIPAL FINDINGS: We established an assay based on two rounds of in vitro restimulation and intracellular cytokine analysis that detects T-cell responses to antigens expressed during latent M. tuberculosis infection. Comparison between active pulmonary tuberculosis (TB) patients and healthy latently M. tuberculosis-infected donors (LTBI) revealed significantly higher T-cell responses against 7 of 35 tested M. tuberculosis latency-associated antigens in LTBI. Notably, T cells specific for Rv3407 were exclusively detected in LTBI but not in TB patients. The T-cell IFNgamma response against Rv3407 in individual donors was the most influential factor in discrimination analysis that classified TB patients and LTBI with 83% accuracy using cross-validation. Rv3407 peptide pool stimulations revealed distinct candidate epitopes in four LTBI. CONCLUSIONS: Our findings further support the hypothesis that the latency-associated antigens can be exploited as biomarkers for LTBI. | | External Publication Status: | published | | Document Type: | Article |
| Communicated by: | Hilmar Fünning | | Affiliations: | MPI für Infektionsbiologie/Department of Immunology
| | External Affiliations: | Respiratory Diseases Clinic Heckeshorn, Department of Pneumology, HELIOS Klinikum Emil von Behring, Berlin, Germany; Asklepios Professional Clinic München-Gauting, Centre for Pneumology and Thorax Surgery, Munich, Germany; Department of Immunohematology & Blood Transfusion/Department of Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands; Research Institute for the Biology of Farm Animals, Genetics and Biometry, Dummerstorf, Germany
| | Identifiers: | ISSN:1932-6203 [Electronic] | |
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