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          Institute: MPI für Infektionsbiologie     Collection: Department of Immunology     Display Documents



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ID: 444325.0, MPI für Infektionsbiologie / Department of Immunology
Identification of T-cell antigens specific for latent mycobacterium tuberculosis infection.
Authors:Schuck, Sebastian D.; Mueller, Henrik; Kunitz, Frank; Neher, Albert; Hoffmann, Harald; Franken, Kees L. C. M.; Repsilber, Dirk; Ottenhoff, Tom H. M.; Kaufmann, Stefan H. E.; Jacobsen, Marc
Language:English
Date of Publication (YYYY-MM-DD):2009-05
Title of Journal:PLoS ONE
Volume:4
Issue / Number:5
Sequence Number of Article:e5590
Copyright:© 2009 Schuck et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Review Status:not specified
Audience:Experts Only
Abstract / Description:BACKGROUND: T-cell responses against dormancy-, resuscitation-, and reactivation-associated antigens of Mycobacterium tuberculosis are candidate biomarkers of latent infection in humans. METHODOLOGY/PRINCIPAL FINDINGS: We established an assay based on two rounds of in vitro restimulation and intracellular cytokine analysis that detects T-cell responses to antigens expressed during latent M. tuberculosis infection. Comparison between active pulmonary tuberculosis (TB) patients and healthy latently M. tuberculosis-infected donors (LTBI) revealed significantly higher T-cell responses against 7 of 35 tested M. tuberculosis latency-associated antigens in LTBI. Notably, T cells specific for Rv3407 were exclusively detected in LTBI but not in TB patients. The T-cell IFNgamma response against Rv3407 in individual donors was the most influential factor in discrimination analysis that classified TB patients and LTBI with 83% accuracy using cross-validation. Rv3407 peptide pool stimulations revealed distinct candidate epitopes in four LTBI. CONCLUSIONS: Our findings further support the hypothesis that the latency-associated antigens can be exploited as biomarkers for LTBI.
External Publication Status:published
Document Type:Article
Communicated by:Hilmar Fünning
Affiliations:MPI für Infektionsbiologie/Department of Immunology
External Affiliations:Respiratory Diseases Clinic Heckeshorn, Department of Pneumology, HELIOS Klinikum Emil von Behring, Berlin, Germany; Asklepios Professional Clinic München-Gauting, Centre for Pneumology and Thorax Surgery, Munich, Germany; Department of Immunohematology & Blood Transfusion/Department of Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands; Research Institute for the Biology of Farm Animals, Genetics and Biometry, Dummerstorf, Germany
Identifiers:ISSN:1932-6203 [Electronic]
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