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          Institute: MPI für molekulare Genetik     Collection: Department of Vertebrate Genomics     Display Documents

ID: 444330.0, MPI für molekulare Genetik / Department of Vertebrate Genomics
Genetic and functional properties of the self-transmissible Yersinia enterocolitica plasmid pYE854, which mobilizes the virulence plasmid pYV
Authors:Hammerl, Jens A.; Klein, Iris; Lanka, Erich; Appel, Bernd; Hertwig, Stefan
Date of Publication (YYYY-MM-DD):2008-02-01
Title of Journal:Journal of Bacteriology
Journal Abbrev.:J Bacteriol
Issue / Number:3
Start Page:991
End Page:1010
Copyright:© 2008, American Society for Microbiology. All Rights Reserved.
Review Status:not specified
Audience:Experts Only
Abstract / Description:Yersinia strains frequently harbor plasmids, of which the virulence plasmid pYV, indigenous in pathogenic strains, has been thoroughly characterized during the last decades. Yet, it has been unknown whether the nonconjugative pYV can be transferred by helper plasmids naturally occurring in this genus. We have isolated the conjugative plasmids pYE854 (95.5 kb) and pYE966 (70 kb) from a nonpathogenic and a pathogenic Yersinia enterocolitica strain, respectively, and demonstrate that both plasmids are able to mobilize pYV. The complete sequence of pYE854 has been determined. The transfer proteins and oriT of the plasmid reveal similarities to the F factor. However, the pYE854 replicon does not belong to the IncF group and is more closely related to a plasmid of gram-positive bacteria. Plasmid pYE966 is very similar to pYE854 but lacks two DNA regions of the larger plasmid that are dispensable for conjugation.
Comment of the Author/Creator:Corresponding author. Mailing address: Bundesinstitut für Risikobewertung, Molekulare Diagnostik und Genetik, Diedersdorfer Weg 1, D-12277 Berlin, Germany. Phone: 49-30-8412-2019. Fax: 49-30-8412-2000. E-mail: stefan.hertwig@bfr.bund.de
Published ahead of print on 30 November 2007.

Supplemental material for this article may be found at http://jb.asm.org/.
External Publication Status:published
Document Type:Article
Communicated by:Hans Lehrach
Affiliations:MPI für molekulare Genetik
External Affiliations:Bundesinstitut für Risikobewertung, D-12277 Berlin, Germany;
Robert Koch-Institut, D-13353 Berlin, Germany.
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