Home News About Us Contact Contributors Disclaimer Privacy Policy Help FAQ

Quick Search
My eDoc
Session History
Support Wiki
Direct access to
document ID:

          Institute: MPI für molekulare Genetik     Collection: Research Group Development and Disease     Display Documents

ID: 447285.0, MPI für molekulare Genetik / Research Group Development and Disease
PBX1 is dispensable for neural commitment of RA-treated murine ES cells
Authors:Jürgens, Anne S.; Kolanczyk, Mateusz; Moebest, Dietrich C. C.; Zemojtel, Tomasz; Lichtenauer, Urs; Duchniewicz, Marlena; Gantert, Melanie P.; Hecht, Jochen; Hattenhorst, Uwe; Burdach, Stefan; Dorn, Annette; Kamps, Mark P.; Beuschlein, Felix; Räpple, Daniel; Scheele, Jürgen S.
Date of Publication (YYYY-MM-DD):2009-05
Title of Journal:In Vitro Cellular & Developmental Biology - Animal
Journal Abbrev.:In Vitro Cell Dev Biol Anim
Issue / Number:5-6
Start Page:252
End Page:263
Copyright:© Springer. Part of Springer Science+Business Media
Review Status:not specified
Audience:Experts Only
Abstract / Description:Experimentation with PBX1 knockout mice has shown that PBX1 is necessary for early embryogenesis. Despite broad insight into PBX1 function, little is known about the underlying target gene regulation. Utilizing the Cre–loxP system, we targeted a functionally important part of the homeodomain of PBX1 through homozygous deletion of exon-6 and flanking intronic regions leading to exon 7 skipping in embryonic stem (ES) cells. We induced in vitro differentiation of wild-type and PBX1 mutant ES cells by aggregation and retinoic acid (RA) treatment and compared their profiles of gene expression at the ninth day post-reattachment to adhesive media. Our results indicate that PBX1 interactions with HOX proteins and DNA are dispensable for RA-induced ability of ES to express neural genes and point to a possible involvement of PBX1 in the regulation of imprinted genes.
Free Keywords:PBX1; Differentiation; ES cells; Imprinting; Expression profiling
Comment of the Author/Creator:email: Juergen.scheele@uniklinik-freiburg.de
External Publication Status:published
Document Type:Article
Communicated by:Stefan Mundlos
Affiliations:MPI für molekulare Genetik
External Affiliations:Department of Medicine I, University of Freiburg Medical Center, Hugstetter Str. 55, 79106 Freiburg, Germany
Department of Pediatrics and Biocenter, University of Halle, Ernst-Grube-Str. 40, 06120 Halle (Saale), Germany
Department of Pathology, University of California, San Diego, School of Medicine, 9500 Gilman Drive, La Jolla, CA 92093, USA
Department of Biology III, Institute for Biology, University of Freiburg, Schänzlestr. 1, 79104 Freiburg, Germany
Department of Bioinformatics, Max-Planck Institute for Molecular Genetics, Ihnestrasse 63-73, 14195 Berlin, Germany
Department of Pharmacology I, University of Freiburg Medical Center, Albertstr. 25, 79106 Freiburg, Germany
Department of Medicine II, University of Freiburg Medical Center, Hugstetter Str. 55, 79106 Freiburg, Germany
The scope and number of records on eDoc is subject to the collection policies defined by each institute - see "info" button in the collection browse view.