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          Institute: MPI für molekulare Genetik     Collection: Ribosome Research Groups     Display Documents



  history
ID: 456775.0, MPI für molekulare Genetik / Ribosome Research Groups
Fast biosynthesis of GFP molecules : a single-molecule fluorescence study
Authors:Katranidis, Alexandros; Atta, Diaa; Schlesinger, Ramona; Nierhaus, Knud H.; Choli-Papadopoulou, Theodora; Gregor, Ingo; Gerrits, Michael; Büldt, Georg; Fitter, Jörg
Language:English
Date of Publication (YYYY-MM-DD):2009
Title of Journal:Angewandte Chemie International Edition
Journal Abbrev.:Angew Chem Int Ed
Volume:48
Issue / Number:10
Start Page:1758
End Page:1761
Copyright:© 2009 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Review Status:not specified
Audience:Experts Only
Abstract / Description:It's not easy being green: Real-time visualization of labeled ribosomes and de novo synthesized green fluorescent protein molecules using single-molecule-sensitive fluorescence microscopy demonstrates that the mutant GFPem is produced with a characteristic time of five minutes. Fluorescence of the fastest GFP molecules appears within one minute.
Free Keywords:Fluorescence microscopy, Green fluorescent proteins; Protein folding; Ribosomes; Single-molecule studies
Comment of the Author/Creator:email: Georg Büldt (g.bueldt@fz-juelich.de)
Jörg Fitter (j.fitter@fz-juelich.de)
External Publication Status:published
Document Type:Article
Communicated by:Ribosome Group
Affiliations:MPI für molekulare Genetik
External Affiliations:Forschungszentrum Jülich, ISB-2: Molecular Biophysics, 52425 Jülich (Germany)
Laboratory of Biochemistry, Aristotle University of Thessaloniki (Greece)
Forschungszentrum Jülich, ISB-1: Cellular Biophysics, Jülich (Germany)
RiNA GmbH, Takustrasse 3, 14195 Berlin (Germany)
Present address: Forschungszentrum caesar, Abt. Molekulare Neurosensorik, Bonn (Germany)
Identifiers:DOI:10.1002/anie.200806070
ISSN:1433-7851
URL:http://www3.interscience.wiley.com/cgi-bin/fulltex...
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