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          Institute: MPI für molekulare Genetik     Collection: Ribosome Research Groups     Display Documents

ID: 457054.0, MPI für molekulare Genetik / Ribosome Research Groups
EF-G2mt is an exclusive recycling factor in mammalian mitochondrial protein synthesis
Authors:Tsuboi, Masafumi; Morita, Hiroyuki; Nozaki, Yusuke; Akama, Kenta; Ueda, Takuya; Ito, Koichi; Nierhaus, Knud H.; Takeuchi, Nono
Date of Publication (YYYY-MM-DD):2009-08-28
Title of Journal:Molecular Cell
Journal Abbrev.:Mol Cell
Issue / Number:4
Start Page:502
End Page:510
Copyright:© 2009 Elsevier B.V.
Review Status:not specified
Audience:Experts Only
Abstract / Description:Bacterial translation elongation factor G (EF-G) catalyzes translocation during peptide elongation and mediates ribosomal disassembly during ribosome recycling in concert with the ribosomal recycling factor (RRF). Two homologs of EF-G have been identified in mitochondria from yeast to man, EF-G1mt and EF-G2mt. Here, we demonstrate that the dual function of bacterial EF-G is divided between EF-G1mt and EF-G2mt in human mitochondria (RRFmt). EF-G1mt specifically catalyzes translocation, whereas EF-G2mt mediates ribosome recycling with human mitochondrial RRF but lacks translocation activity. Domain swapping experiments suggest that the functional specificity for EF-G2mt resides in domains III and IV. Furthermore, GTP hydrolysis by EF-G2mt is not necessary for ribosomal splitting, in contrast to the bacterial-recycling mode. Because EF-G2mt represents a class of translational GTPase that is involved in ribosome recycling, we propose to rename this factor mitochondrial ribosome recycling factor 2 (RRF2mt).
Free Keywords:Proteins; RNA
Comment of the Author/Creator:Referred to by:
Ribosome Shifting or Splitting: It Is All Up To the EF-G
Molecular Cell, Volume 35, Issue 4, 28 August 2009, Pages 400-402, Brooke Christian, Emdadul Haque, Linda Spremulli
External Publication Status:published
Document Type:Article
Communicated by:Ribosome Group
Affiliations:MPI für molekulare Genetik
External Affiliations:Department of Medical Genome Sciences, Graduate School of Frontier Sciences, University of Tokyo, 5-1-5, Kashiwanoha, Kashiwa-shi, Chiba 277-8562, Japan
Department of Basic Medical Sciences, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan
Precursory Research for Embryonic Science and Technology, Japan Science and Technology Agency, 4-1-8 Honcho, Kawaguchi-shi, Saitama 332-0012, Japan
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