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          Institute: MPI für molekulare Genetik     Collection: Independent Junior Research Groups (Otto-Warburg-Laboratory)     Display Documents



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ID: 457093.0, MPI für molekulare Genetik / Independent Junior Research Groups (Otto-Warburg-Laboratory)
Transcriptome analysis by strand-specific sequencing of complementary DNA
Authors:Parkhomchuk, Dmitri; Borodina, Tatiana; Amstislavskiy, Vyacheslav; Banaru, Maria; Hallen, Linda; Krobitsch, Sylvia; Lehrach, Hans; Soldatov, Alexey
Language:English
Date of Publication (YYYY-MM-DD):2009-10
Title of Journal:Nucleic Acids Research
Journal Abbrev.:NAR
Volume:37
Issue / Number:18
Start Page:e123
End Page:e123
Copyright:© 2009 The Author(s)
Review Status:not specified
Audience:Experts Only
Abstract / Description:High-throughput complementary DNA sequencing (RNA-Seq) is a powerful tool for whole-transcriptome analysis, supplying information about a transcript's expression level and structure. However, it is difficult to determine the polarity of transcripts, and therefore identify which strand is transcribed. Here, we present a simple cDNA sequencing protocol that preserves information about a transcript's direction. Using Saccharomyces cerevisiae and mouse brain transcriptomes as models, we demonstrate that knowing the transcript's orientation allows more accurate determination of the structure and expression of genes. It also helps to identify new genes and enables studying promoter-associated and antisense transcription. The transcriptional landscapes we obtained are available online.
Comment of the Author/Creator:email: soldatov@molgen.mpg.de
External Publication Status:published
Document Type:Article
Communicated by:OWL-Group
Affiliations:MPI für molekulare Genetik
Identifiers:DOI:10.1093/nar/gkp596
ISSN:0305-1048
URL:http://nar.oxfordjournals.org/cgi/reprint/37/18/e1...
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