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          Institute: MPI für Entwicklungsbiologie     Collection: Abteilung 6 - Molecular Biology (D. Weigel)     Display Documents



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ID: 460669.0, MPI für Entwicklungsbiologie / Abteilung 6 - Molecular Biology (D. Weigel)
Deep sequencing to reveal new variants in pooled DNA samples
Authors:Out, A. A.; van Minderhout, I. J.; Goeman, J. J.; Ariyurek, Y.; Ossowski, S.; Schneeberger, K.; Weigel, D.; van Galen, M.; Taschner, P. E.; Tops, C. M.; Breuning, M. H.; van Ommen, G. J.; den Dunnen, J. T.; Devilee, P.; Hes, F. J.
Date of Publication (YYYY-MM-DD):2009-12
Title of Journal:Hum Mutat
Volume:30
Issue / Number:12
Start Page:1703
End Page:1712
Review Status:not specified
Audience:Not Specified
Abstract / Description:We evaluated massive parallel sequencing and long-range PCR (LRP) for rare variant detection and allele frequency estimation in pooled DNA samples. Exons 2 to 16 of the MUTYH gene were analyzed in breast cancer patients with Illumina's (Solexa) technology. From a pool of 287 genomic DNA samples we generated a single LRP product, while the same LRP was performed on 88 individual samples and the resulting products then pooled. Concentrations of constituent samples were measured with fluorimetry for genomic DNA and high-resolution melting curve analysis (HR-MCA) for LRP products. Illumina sequencing results were compared to Sanger sequencing data of individual samples. Correlation between allele frequencies detected by both methods was poor in the first pool, presumably because the genomic samples amplified unequally in the LRP, due to DNA quality variability. In contrast, allele frequencies correlated well in the second pool, in which all expected alleles at a frequency of 1% and higher were reliably detected, plus the majority of singletons (0.6% allele frequency). We describe custom bioinformatics and statistics to optimize detection of rare variants and to estimate required sequencing depth. Our results provide directions for designing high-throughput analyses of candidate genes.
External Publication Status:published
Document Type:Article
Affiliations:MPI für Entwicklungsbiologie/Abteilung 6 - Molekulare Biologie (Detlef Weigel)
External Affiliations:%G eng
Identifiers:ISSN:1098-1004 (Electronic) 1098-1004 (Linking) %R 10.1... [ID No:1]
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=... [ID No:2]
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