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          Institute: MPI für molekulare Genetik     Collection: Sequencing Group     Display Documents

ID: 461116.0, MPI für molekulare Genetik / Sequencing Group
Toward Cloning of the Magnetotactic Metagenome: Identification of Magnetosome Island Gene Clusters in Uncultivated Magnetotactic Bacteria from Different Aquatic Sediments.
Authors:Jogler, Christian; Lin, Wei; Meyerdierks, Anke; Kube, Michael; Katzmann, Emanuel; Flies, Christine; Pan, Yongxin; Amann, Rudolf; Reinhard, Richard; Schüler, Dirk
Date of Publication (YYYY-MM-DD):2009-04-24
Title of Journal:Applied and Environmental Microbiology
Journal Abbrev.:Appl. Environ. Microbiol.
Issue / Number:12
Start Page:3972
End Page:3979
Copyright:2009, American Society for Microbiology. All Rights Reserved.
Review Status:not specified
Audience:Experts Only
Abstract / Description:In this report, we describe the selective cloning of large DNA fragments from magnetotactic metagenomes from various aquatic habitats. This was achieved by a two-step magnetic enrichment which allowed the mass collection of environmental magnetotactic bacteria (MTB) virtually free of nonmagnetic contaminants. Four fosmid libraries were constructed and screened by end sequencing and hybridization analysis using heterologous magnetosome gene probes. A total of 14 fosmids were fully sequenced. We identified and characterized two fosmids, most likely originating from two different alphaproteobacterial strains of MTB that contain several putative operons with homology to the magnetosome island (MAI) of cultivated MTB. This is the first evidence that uncultivated MTB exhibit similar yet differing organizations of the MAI, which may account for the diversity in biomineralization and magnetotaxis observed in MTB from various environments.
Comment of the Author/Creator:Corresponding author.
Mailing address: LMU Department Biology I, Microbiology, Großharderner-Str. 2, 82152 Planegg-Martinsried, Germany. Phone: 49 (0)89-2180-74502. Fax: 49 (0)89-2180-74515. E-mail: dschuele@mpi-bremen.de
External Publication Status:published
Document Type:Article
Communicated by:Richard Reinhardt
Affiliations:MPI für molekulare Genetik
MPI für marine Mikrobiologie
External Affiliations:1.LMU Department Biology I, Microbiology, Großharderner-Str. 2, 82152 Planegg-Martinsried, Germany;
2.Biogeomagnetism Group, Paleomagnetism and Geochronology Laboratory, State Key Laboratory of Lithospheric Evolution, Institute of Geology and Geophysics, Chinese Academy of Sciences, Beijing 100029, China.
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