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          Institute: MPI für molekulare Zellbiologie und Genetik     Collection: Publikationen MPI-CBG 2009 arch     Display Documents



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ID: 463107.0, MPI für molekulare Zellbiologie und Genetik / Publikationen MPI-CBG 2009 arch
The mitotic kinesin-14 Ncd drives directional microtubule-microtubule sliding
Authors:Fink, Gero; Hajdo, Lukasz; Skowronek, Krzysztof J; Reuther, Cordula; Kasprzak, Andrzej A; Diez, Stefan
Date of Publication (YYYY-MM-DD):2009
Title of Journal:Nature Cell Biology
Volume:11
Issue / Number:6
Start Page:717
End Page:723
Copyright:not available
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:During mitosis and meiosis, the bipolar spindle facilitates chromosome segregation through microtubule sliding as well as microtubule growth and shrinkage. Kinesin-14, one of the motors involved, causes spindle collapse in the absence of kinesin-5 (Refs 2, 3), participates in spindle assembly and modulates spindle length. However, the molecular mechanisms underlying these activities are not known. Here, we report that Drosophila melanogaster kinesin-14 (Ncd) alone causes sliding of anti-parallel microtubules but locks together (that is, statically crosslinks) those that are parallel. Using single molecule imaging we show that Ncd diffuses along microtubules in a tail-dependent manner and switches its orientation between sliding microtubules. Our results show that kinesin-14 causes sliding and expansion of an anti-parallel microtubule array by dynamic interactions through the motor domain on the one side and the tail domain on the other. This mechanism accounts for the roles of kinesin-14 in spindle organization.
External Publication Status:published
Document Type:Article
Version Comment:Automatic journal name synchronization
Communicated by:n.n.
Affiliations:MPI für molekulare Zellbiologie und Genetik
Identifiers:LOCALID:1212
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