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          Institute: MPI für molekulare Zellbiologie und Genetik     Collection: Publikationen MPI-CBG 2009 arch     Display Documents



ID: 463165.0, MPI für molekulare Zellbiologie und Genetik / Publikationen MPI-CBG 2009 arch
Reconstitution of Rab- and SNARE-dependent membrane fusion by synthetic endosomes
Authors:Ohya, Takeshi; Miaczynska, Marta; Coskun, Ünal; Lommer, Barbara; Runge, Anja; Drechsel, David; Kalaidzidis, Yannis; Zerial, Marino
Date of Publication (YYYY-MM-DD):2009
Title of Journal:Nature
Volume:459
Issue / Number:7250
Start Page:1091
End Page:1097
Copyright:not available
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:Rab GTPases and SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) are evolutionarily conserved essential components of the eukaryotic intracellular transport system. Although pairing of cognate SNAREs is sufficient to fuse membranes in vitro, a complete reconstitution of the Rab-SNARE machinery has never been achieved. Here we report the reconstitution of the early endosomal canine Rab5 GTPase, its key regulators and effectors together with SNAREs into proteoliposomes using a set of 17 recombinant human proteins. These vesicles behave like minimal 'synthetic' endosomes, fusing with purified early endosomes or with each other in vitro. Membrane fusion measured by content-mixing and morphological assays requires the cooperativity between Rab5 effectors and cognate SNAREs which, together, form a more efficient 'core machinery' than SNAREs alone. In reconstituting a fusion mechanism dependent on both a Rab GTPase and SNAREs, our work shows that the two machineries act coordinately to increase the specificity and efficiency of the membrane tethering and fusion process.
External Publication Status:published
Document Type:Article
Communicated by:n.n.
Affiliations:MPI für molekulare Zellbiologie und Genetik
Identifiers:LOCALID:1240
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