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          Institute: MPI für molekulare Zellbiologie und Genetik     Collection: Publikationen MPI-CBG 2009 arch     Display Documents



ID: 463168.0, MPI für molekulare Zellbiologie und Genetik / Publikationen MPI-CBG 2009 arch
HAUS, the 8-subunit human Augmin complex, regulates centrosome and spindle integrity
Authors:Lawo, Steffen; Bashkurov, Mikhail; Mullin, Michael; Ferreria, Mariana Gomez; Kittler, Ralf; Habermann, Bianca; Tagliaferro, Andrea; Poser, Ina; Hutchins, James R A; Hegemann, Bjorn; Pinchev, Deborah; Buchholz, Frank; Peters, Jan-Michael; Hyman, Anthony A; Gingras, Anne-Claude; Pelletier, Laurence
Date of Publication (YYYY-MM-DD):2009
Title of Journal:Curr Biol
Volume:19
Issue / Number:10
Start Page:816
End Page:826
Copyright:not available
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:BACKGROUND: The assembly of a robust microtubule-based mitotic spindle is a prerequisite for the accurate segregation of chromosomes to progeny. Spindle assembly relies on the concerted action of centrosomes, spindle microtubules, molecular motors, and nonmotor spindle proteins. RESULTS: Here we use an RNA-interference screen of the human centrosome proteome to identify novel regulators of spindle assembly. One such regulator is HAUS, an 8-subunit protein complex that shares homology to Drosophila Augmin. HAUS localizes to interphase centrosomes and to mitotic spindle microtubules, and its disruption induces microtubule-dependent fragmentation of centrosomes along with an increase in centrosome size. HAUS disruption results in the destabilization of kinetochore microtubules and the eventual formation of multipolar spindles. These severe mitotic defects are alleviated by codepletion of NuMA, indicating that both factors regulate opposing activities. HAUS disruption alters NuMA localization, suggesting that mislocalized NuMA activity contributes to the spindle and centrosome defects observed. CONCLUSION: The human Augmin complex (HAUS) is a critical and evolutionary conserved multisubunit protein complex that regulates centrosome and spindle integrity.
External Publication Status:published
Document Type:Article
Communicated by:n.n.
Affiliations:MPI für molekulare Zellbiologie und Genetik
Identifiers:LOCALID:1243
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