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          Institute: MPI für molekulare Zellbiologie und Genetik     Collection: Publikationen MPI-CBG 2009 arch     Display Documents



ID: 463258.0, MPI für molekulare Zellbiologie und Genetik / Publikationen MPI-CBG 2009 arch
Live cell multicolor imaging of lipid droplets with a new dye, LD540
Authors:Spandl, Johanna; White, Daniel J; Peychl, Jan; Thiele, Christoph
Date of Publication (YYYY-MM-DD):2009
Title of Journal:Traffic
Volume:10
Issue / Number:11
Start Page:1579
End Page:1584
Copyright:not available
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:A lipophilic dye based on the Bodipy fluorophore, LD540, was developed for microscopic imaging of lipid droplets. In contrast to previous lipid droplet dyes, it can spectrally be resolved from both green and red fluorophores allowing multicolor imaging in both fixed and living cells. Its improved specificity, brightness and photostability support live cell imaging, which was used to demonstrate by two-color imaging lipid droplet motility along microtubules.
External Publication Status:published
Document Type:Article
Communicated by:n.n.
Affiliations:MPI für molekulare Zellbiologie und Genetik
Identifiers:LOCALID:1414
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