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          Institute: MPI für molekulare Zellbiologie und Genetik     Collection: Publikationen MPI-CBG 2009 arch     Display Documents



ID: 463262.0, MPI für molekulare Zellbiologie und Genetik / Publikationen MPI-CBG 2009 arch
Electron tomography of microtubule end-morphologies in C. elegans embryos
Authors:O'Toole, Eileen; Muller-Reichert, Thomas
Place of Publication:New York
Publisher:Humana Press
Date of Publication (YYYY-MM-DD):2009
Title of Book:Mitosis : methods and protocols
Start Page:135
End Page:144
Sequence Number of Inbook:8
Full Name of Book-Editor(s):Andrew D. McAinsh
Title of Series:Methods in molecular biology ; 545
Copyright:not available
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:In this chapter we describe the preparation of early mitotic C. elegans embryos for the tomographic reconstruction of end-morphologies of spindle microtubules. Early embryos are prepared by high-pressure freezing and freeze-substitution for thin-layer embedding in Epon/Araldite. We further describe data acquisition, tomographic reconstruction, and 3-D modeling of microtubules in serially sectioned mitotic spindles. The presented techniques are applicable to other model systems.
External Publication Status:published
Document Type:InBook
Communicated by:n.n.
Affiliations:MPI für molekulare Zellbiologie und Genetik
Identifiers:LOCALID:1418
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