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          Institute: MPI für terrestrische Mikrobiologie     Collection: Department-independent groups     Display Documents

ID: 463326.0, MPI für terrestrische Mikrobiologie / Department-independent groups
Acidianus, Sulfolobus and Metallosphaera surface layers:
structure, composition and gene expression
Authors:Veith, A.; Klingl, A.; Zolghadr, B.; Lauber, K.; Mentele, R.; Lottspeich, F.; Rachel, R.; Albers, S.; Kletzin, A.
Date of Publication (YYYY-MM-DD):2009-06-06
Title of Journal:Molecular Microbiology
Start Page:58
End Page:72
Review Status:not specified
Audience:Not Specified
Abstract / Description:The cell walls of Sulfolobales species consist of proteinaceous
S-layers assembled from two polypeptides,
SlaA and SlaB. We isolated the large S-layer
protein of Acidianus ambivalens and both S-layer
subunits of Sulfolobus solfataricus and Metallosphaera
sedula, respectively. The slaAB genes,
lying adjacently in the chromosomes, are constitutively
transcribed as bicistronic operons in
A. ambivalens and S. solfataricus. A smaller slaA
transcript appeared in Northern hybridizations of
A. ambivalens RNA. PCRs experiments showed that
80–85% of the transcripts stop at an oligo-T terminator
downstream of slaA while 15–20% are read
through to a second terminator downstream of slaB.
The bicistronic operons including promoter and terminator
regions are conserved in the Sulfolobales.
While no SlaA homologue is found outside the Sulfolobales,
SlaB is distantly similar to S-layer proteins
of other Crenarchaeota, e.g. the Staphylothermus
marinus tetrabrachion. Molecular modelling suggests
SlaBs to be composed of 2–3 consecutive beta sandwich
domains, a coiled-coil domain of 15–17 nm in
length and a C-terminal transmembrane helix. Electron
microscopy shows crystalline protein arrays with
triangular and hexagonal pores. We propose that the
mushroom-shaped ‘unit cells’ of the Sulfolobales’
S-layers consist of three SlaBs anchoring the
complex in the membrane and six SlaAs forming the
detergent-resistant outer sacculus.
External Publication Status:published
Document Type:Article
Communicated by:N.N.
Affiliations:MPI für terrestrische Mikrobiologie/Department-independent groups
External Affiliations:Institute of Microbiology and Genetics, Technische
Universität Darmstadt, Schnittspahnstraße 10, 64287
Darmstadt, Germany.

Center for Electron Microscopy, and Department of
Microbiology, Faculty of Natural Sciences III, Universität
Regensburg, Universitätsstraße 31, 93053 Regensburg,

Max-Planck Institute of Biochemistry, Am Klopferspitz
18, 82152 Martinsried, Germany.
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