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          Institute: MPI für medizinische Forschung     Collection: Abteilung Biomedizinische Optik     Display Documents



ID: 473248.0, MPI für medizinische Forschung / Abteilung Biomedizinische Optik
Eyecup Scope − Optical recordings of light stimulus−evoked fluorescence signals in the retina
Translation of Title:Eyecup Scope − Optical recordings of light stimulus−evoked fluorescence signals in the retina
Authors:Euler, Thomas; Hausselt, Susanne; Margolis, David J.; Breuninger, Tobias; Castell, Xavier; Detwiler, Peter B.; Denk, Winfried
Language:English
Date of Publication (YYYY-MM-DD):2009-04-01
Title of Journal:Pfluegers Archiv European Journal of Physiology
Journal Abbrev.:Pfluegers Arch.
Volume:457
Issue / Number:6
Start Page:1393
End Page:1414
Review Status:Peer-review
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:Dendritic signals play an essential role in processing visual information in the retina. To study them in neurites too small for electrical recording, we developed an instrument that combines a multi−photon (MP) microscope with a through−the−objective high−resolution visual stimulator. An upright microscope was designed that uses the objective lens for both MP imaging and delivery of visual stimuli to functionally intact retinal explants or eyecup preparations. The stimulator consists of a miniature liquid−crystal−on−silicon display coupled into the optical path of an infrared−excitation laser−scanning microscope. A pair of custom−made dichroic filters allows light from the excitation laser and three spectral bands (‘colors') from the stimulator to reach the retina, leaving two intermediate bands for fluorescence imaging. Special optics allow displacement of the stimulator focus relative to the imaging focus. Spatially resolved changes in calcium−indicator fluorescence in response to visual stimuli were recorded in dendrites of different types of mammalian retinal neurons.
Free Keywords:Two−photon microscopy; Optical recordings; Visual stimulation; Calciumimaging; Retinal signal processing; Dendritic processing
Last Change of the Resource (YYYY-MM-DD):--
External Publication Status:published
Document Type:Article
Communicated by:Wulf Kaiser
Affiliations:MPI für medizinische Forschung/Abteilung Biomedizinische Optik
Identifiers:LOCALID:7225
URI:http%3A%2F%2Fspringer.metapress.com%2Fcontent%2Fh1...
URI:http%3A%2F%2Fspringer.metapress.com%2Fcontent%2Fh1...
DOI:10.1007%2Fs00424-008-0603-5
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