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          Institute: MPI für medizinische Forschung     Collection: Abteilung Molekulare Neurobiologie     Display Documents



ID: 473364.0, MPI für medizinische Forschung / Abteilung Molekulare Neurobiologie
Split−Cre complementation indicates coincident activity of different genes in vivo
Translation of Title:Split−Cre complementation indicates coincident activity of different genes in vivo
Authors:Hirrlinger, Johannes; Scheller, Anja; Hirrlinger, Petra G.; Kellert, Beate; Tang, Wannan; Wehr, Michael C.; Goebbels, Sandra; Reichenbach, Andreas; Sprengel, Rolf; Rossner, Moritz J.; Kirchhoff, Frank
Language:English
Date of Publication (YYYY-MM-DD):2009-01-27
Title of Journal:PLoS ONE
Journal Abbrev.:PLoS ONE
Volume:4
Issue / Number:1
Start Page:4286
End Page:10
Review Status:Peer-review
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:Cre/LoxP recombination is the gold standard for conditional gene regulation in mice in vivo. However, promoters driving the expression of Cre recombinase are often active in a wide range of cell types and therefore unsuited to target more specific subsets of cells. To overcome this limitation, we designed inactive "split−Cre" fragments that regain Cre activity when overlapping co−expression is controlled by two different promoters. Using transgenic mice and virus−mediated expression of split−Cre, we show that efficient reporter gene activation is achieved in vivo. In the brain of transgenic mice, we genetically defined a subgroup of glial progenitor cells in which the Plp1− and the Gfap−promoter are simultaneously active, giving rise to both astrocytes and NG2−positive glia. Similarly, a subset of interneurons was labelled after viral transfection using Gad67− and Cck1 promoters to express split−Cre. Thus, split−Cre mediated genomic recombination constitutes a powerful spatial and temporal coincidence detector for in vivo targeting.
Last Change of the Resource (YYYY-MM-DD):--
External Publication Status:published
Document Type:Article
Communicated by:Wulf Kaiser
Affiliations:MPI für medizinische Forschung/Abteilung Molekulare Neurobiologie
Identifiers:LOCALID:7285
URI:http%3A%2F%2Fwww.plosone.org%2Farticle%2FfetchObje...
URI:http%3A%2F%2Fwww.plosone.org%2Farticle%2Finfo%253A...
DOI:10.1371%2Fjournal.pone.0004286
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