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          Institute: MPI für medizinische Forschung     Collection: Abteilung Molekulare Neurobiologie     Display Documents



ID: 473391.0, MPI für medizinische Forschung / Abteilung Molekulare Neurobiologie
Fluorescent Arc/Arg3.1 indicator mice: a versatile tool to study brain activity changes in vitro and in vivo
Translation of Title:Fluorescent Arc/Arg3.1 indicator mice: a versatile tool to study brain activity changes in vitro and in vivo
Authors:Grinevich, Valery; Kolleker, Aleksandre; Eliava, M.; Takada, N.; Takuma, Hiroshi; Fukuzawa, Y.; Shigemoto, R.; Kuhl, D.; Waters, David Jack; Seeburg, Peter H.; Osten, Pavel
Language:English
Date of Publication (YYYY-MM-DD):2009-10-30
Title of Journal:Journal of Neuroscience Methods
Journal Abbrev.:J. Neurosci. Methods
Volume:184
Issue / Number:1
Start Page:25
End Page:26
Review Status:Peer-review
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:The brain−specificimmediate early gene Arc/Arg3.1 is induced in response to a variety of stimuli, including
sensory and behavior−linked neural activity. Here we report the generation of transgenic mice, termed
TgArc/Arg3.1−d4EGFP, expressing a 4−h half−life form of enhanced green fluorescent protein (d4EGFP)
under the control of the Arc/Arg3.1 promoter. We show that d4EGFP−mediated fluorescence faithfully
reports Arc/Arg3.1 induction in response to physiological, pathological and pharmacological stimuli, and
that this fluorescence permits electrical recording fromactivated neurons in the live mouse. Moreover, the
fluorescent Arc/Arg3.1 indicator revealed activity changes in circumscribed brain areas in distinct modes
of stress and in a mouse model of Alzheimer's disease. These findings identify the TgArc/Arg3.1−d4EGFP
mouse as a versatile tool to monitor Arc/Arg3.1 induction in neural circuits, both in vitro and in vivo.
Free Keywords:Immediate early genes
Arc
Arg3.1
Green fluorescent protein (GFP)
Transgenic mice
Synaptic plasticity
Neural circuits
Stress
Last Change of the Resource (YYYY-MM-DD):--
External Publication Status:published
Document Type:Article
Communicated by:Wulf Kaiser
Affiliations:MPI für medizinische Forschung/Abteilung Zellphysiologie
MPI für medizinische Forschung/Abteilung Molekulare Neurobiologie
Identifiers:LOCALID:7504
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