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          Institute: MPI für Biophysik     Collection: Abt. Biophysikalische Chemie     Display Documents



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ID: 473595.0, MPI für Biophysik / Abt. Biophysikalische Chemie
Effects of α-Asarone on the Glutamate Transporter EAAC1 in Xenopus Oocytes
Authors:Gu, Quanbao; Du, Huiming; Ma, Chunhui; Fotis, Heike; Wu, Bin; Huang, Chenggang; Schwarz, Wolfgang
Language:English
Date of Publication (YYYY-MM-DD):2009-01
Title of Journal:Planta Medica
Journal Abbrev.:Planta Med.
Volume:75
Issue / Number:1
Start Page:1
End Page:4
Review Status:Peer-review
Audience:Experts Only
Abstract / Description:The major excitatory neurotransmitter transporter EAAC1 in the mammalian central nervous system is considered a possible target for Chinese herbal medicine. Extracts of Acorus tatarinowii (Schott) were tested for their effects on EAAC1 activity. Xenopus oocytes with heterologously expressed EAAC1 were used as the model system. Rate of glutamate uptake was determined by means of the isotopic tracer technique. Glutamate-induced current was recorded under a two-electrode voltage clamp. As a highly effective component, α-asarone was identified. The rate of glutamate uptake was stimulated by 200 µM of α-asarone by about 15 %. In contrast, the same concentration reduced the EAAC1-mediated current by about 35 % at a holding potential of − 60 mV; half maximum inhibition was obtained at about 60 µM. Our experimental data suggest that both stimulation of glutamate uptake and inhibition of EAAC1-mediated current by α-asarone could contribute to reduced excitatory activity.
Free Keywords:Acorus tatarinowi; Acoraceae; α-asarone; neurotransmitter transporter; glutamate; Xenopus oocytes; voltage clamp
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:MPI für Biophysik/Abteilung Biophysikalische Chemie
External Affiliations:Shanghai Research Center for Acupuncture and Meridians, Shanghai, China;
Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China
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