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| ID:
473595.0,
MPI für Biophysik / Abt. Biophysikalische Chemie |
| Effects of α-Asarone on the Glutamate Transporter EAAC1 in Xenopus Oocytes |
| Authors: | Gu, Quanbao; Du, Huiming; Ma, Chunhui; Fotis, Heike; Wu, Bin; Huang, Chenggang; Schwarz, Wolfgang | | Language: | English | | Date of Publication (YYYY-MM-DD): | 2009-01 | | Title of Journal: | Planta Medica | | Journal Abbrev.: | Planta Med. | | Volume: | 75 | | Issue / Number: | 1 | | Start Page: | 1 | | End Page: | 4 | | Review Status: | Peer-review | | Audience: | Experts Only | | Abstract / Description: | The major excitatory neurotransmitter transporter EAAC1 in the mammalian central nervous system is considered a possible target for Chinese herbal medicine. Extracts of Acorus tatarinowii (Schott) were tested for their effects on EAAC1 activity. Xenopus oocytes with heterologously expressed EAAC1 were used as the model system. Rate of glutamate uptake was determined by means of the isotopic tracer technique. Glutamate-induced current was recorded under a two-electrode voltage clamp. As a highly effective component, α-asarone was identified. The rate of glutamate uptake was stimulated by 200 µM of α-asarone by about 15 %. In contrast, the same concentration reduced the EAAC1-mediated current by about 35 % at a holding potential of − 60 mV; half maximum inhibition was obtained at about 60 µM. Our experimental data suggest that both stimulation of glutamate uptake and inhibition of EAAC1-mediated current by α-asarone could contribute to reduced excitatory activity. | | Free Keywords: | Acorus tatarinowi; Acoraceae; α-asarone; neurotransmitter transporter; glutamate; Xenopus oocytes; voltage clamp | | External Publication Status: | published | | Document Type: | Article |
| Communicated by: | N. N. | | Affiliations: | MPI für Biophysik/Abteilung Biophysikalische Chemie
| | External Affiliations: | Shanghai Research Center for Acupuncture and Meridians, Shanghai, China;
Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China
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