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          Institute: MPI für molekulare Genetik     Collection: Microscopy     Display Documents



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ID: 473605.0, MPI für molekulare Genetik / Microscopy
Functional Genomic Analysis of Two Staphylococcus aureus Phages Isolated from the Dairy Environment.
Authors:García, Pilar; Martínez, Beatriz; Obeso, José María; Lavigne, Rob; Lurz, Rudi; Rodríguez, Ana
Language:English
Date of Publication (YYYY-MM-DD):2009-12-01
Title of Journal:Applied and Environmental Microbiology
Journal Abbrev.:Appl. Environ. Microbiol.
Volume:75
Issue / Number:24
Start Page:7663
End Page:7673
Copyright:2009, American Society for Microbiology. All Rights Reserved.
Review Status:not specified
Audience:Experts Only
Abstract / Description:The genomes of the two lytic mutant Staphylococcus aureus bacteriophages, vB_SauS-phiIPLA35 (phiIPLA35) and vB_SauS-phiIPLA88 (phiIPLA88), isolated from milk have been analyzed. Their genomes are 45,344 bp and 42,526 bp long, respectively, and contain 62 and 61 open reading frames (ORFS). Enzymatic analyses and sequencing revealed that the phiIPLA35 DNA molecule has 3'-protruding cohesive ends (cos) 10 bp long, whereas phiIPLA88 DNA is 4.5% terminally redundant and most likely is packaged by a headful mechanism. N-terminal amino acid sequencing, mass spectrometry, bioinformatic analyses, and functional analyses enabled the assignment of putative functions to 58 gene products, including DNA packaging proteins, morphogenetic proteins, lysis components, and proteins necessary for DNA recombination, modification, and replication. Point mutations in their lysogeny control-associated genes explain their strictly lytic behavior. Muralytic activity associated with other structural components has been detected in virions of both phages. Comparative analysis of phiIPLA35 and phiIPLA88 genome structures shows that they resemble those of {phi}12 and {phi}11, respectively, both representatives of large genomic groupings within the S. aureus-infecting phages.
Comment of the Author/Creator:Corresponding author.
Mailing address: Instituto de Productos Lácteos de Asturias, Consejo Superior de Investigaciones Científicas, Apdo. 85, 33300 Villaviciosa, Asturias, Spain. Phone: 34 985 89 21 31. Fax: 34 985 89 22 33. E-mail: pgarcia@ipla.csic.es
External Publication Status:published
Document Type:Article
Communicated by:Rudi Lurz
Affiliations:MPI für molekulare Genetik
External Affiliations:1.Instituto de Productos Lácteos de Asturias, Consejo Superior de Investigaciones Científicas, 33300 Villaviciosa, Asturias, Spain;
2.Division of Gene Technology, Department of Biosystems, Katholieke Universiteit Leuven, Kasteelpark Arenberg 21, B-3001 Leuven, Belgium.
Identifiers:URL:http://aem.asm.org/cgi/content/abstract/75/24/7663
DOI:10.1128/AEM.01864-09
ISSN:0099-2240
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