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          Institute: MPI für Herz- und Lungenforschung (W. G. Kerckhoff Institut)     Collection: Publikationen des W. G. Kerckhoff-Instituts     Display Documents



ID: 474448.0, MPI für Herz- und Lungenforschung (W. G. Kerckhoff Institut) / Publikationen des W. G. Kerckhoff-Instituts
Endotoxin-induced gene expression differences in the brain and effects of iNOS inhibition and norepinephrine
Authors:Wolff, S.; Klatt, S.; Wolff, J. C.; Wilhelm, J.; Fink, L.; Kaps, M.; Rosengarten, B.
Date of Publication (YYYY-MM-DD):2009
Title of Journal:Intensive Care Med
Volume:35
Issue / Number:4
Start Page:730
End Page:9
Audience:Not Specified
Abstract / Description:PURPOSE: We studied gene expression differences in brain homogenate, hippocampus, somatosensory cortex and cerebellum of rats suffering from sepsis-associated delirium and analyzed the effects of norepinephrine and 1,400 W (specific inhibitor of the inducible nitric-oxide synthase). METHODS: We applied microarray screenings to rat brain homogenate 1, 3 and 4.5 h after lipopolysaccharide (LPS, 5 mg/kg) or 0.9% NaCl treatment. Therapy groups were analyzed after 4.5 h. Validations and compartment specific investigations were carried out by real-time PCR. RESULTS: Most striking gene expression differences were seen 4.5 h after LPS administration, especially within the hippocampus (chemokines and endothelial cell-specific molecule 1). Norepinephrine resulted in a discrete chemokine up-regulation, while 1,400 W had hardly any effect. CONCLUSION: Strongest gene regulations were found within the hippocampus. Norepinephrine showed a tendency of having a proinflammatory influence, while 1,400 W had no clear-cut effect onto the gene expression level.
Free Keywords:Animals; Blotting, Western; Chemokines/metabolism; DNA Primers/genetics; Delirium/etiology/metabolism; Gene Expression/*genetics; Hippocampus/*metabolism; Neurotoxicity Syndromes/etiology/metabolism; Neurotoxins/*genetics/*metabolism; Nitric Oxide Synthase/*antagonists & inhibitors; Norepinephrine/*metabolism; Oligonucleotide Array Sequence Analysis; Polymerase Chain Reaction; RNA, Messenger/genetics; Rats; Rats, Sprague-Dawley; Sepsis/complications/metabolism
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:MPI für physiologische und klinische Forschung
External Affiliations:Department of Neurology, Justus-Liebig-University Giessen, Am Steg 14, 35392, Giessen, Germany. Stephanie.Wolff@neuro.med.uni-giessen.de
Identifiers:ISSN:1432-1238 (Electronic) 0342-4642 (Linking)
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=..
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