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          Institute: MPI für Herz- und Lungenforschung (W. G. Kerckhoff Institut)     Collection: Publikationen des W. G. Kerckhoff-Instituts     Display Documents



ID: 474449.0, MPI für Herz- und Lungenforschung (W. G. Kerckhoff Institut) / Publikationen des W. G. Kerckhoff-Instituts
An integrated approach for the systematic identification and characterization of heart-enriched genes with unknown functions
Authors:Uchida, S.; Schneider, A.; Wiesnet, M.; Jungblut, B.; Zarjitskaya, P.; Jenniches, K.; Kreymborg, K. G.; Seeger, W.; Braun, T.
Date of Publication (YYYY-MM-DD):2009
Title of Journal:BMC Genomics
Volume:10
Start Page:100
Audience:Not Specified
Abstract / Description:ABSTRACT: BACKGROUND: High throughput techniques have generated a huge set of biological data, which are deposited in various databases. Efficient exploitation of these databases is often hampered by a lack of appropriate tools, which allow easy and reliable identification of genes that miss functional characterization but are correlated with specific biological conditions (e.g. organotypic expression). RESULTS: We have developed a simple algorithm (DGSA = Database-dependent Gene Selection and Analysis) to identify genes with unknown functions involved in organ development concentrating on the heart. Using our approach, we identified a large number of yet uncharacterized genes, which are expressed during heart development. An initial functional characterization of genes by loss-of-function analysis employing morpholino injections into zebrafish embryos disclosed severe developmental defects indicating a decisive function of selected genes for developmental processes. CONCLUSION: We conclude that DGSA is a versatile tool for database mining allowing efficient selection of uncharacterized genes for functional analysis.
Free Keywords:*Algorithms; Animals; Computational Biology; *Database Management Systems; Databases, Genetic; Gene Expression Profiling/*methods; Gene Expression Regulation, Developmental; Mice; Myocardium/*metabolism; Oligonucleotide Array Sequence Analysis/methods; Sequence Analysis, RNA; Zebrafish/embryology/genetics
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:MPI für physiologische und klinische Forschung
External Affiliations:Max-Planck-Institute for Heart and Lung Research, Parkstrasse 1, Bad Nauheim, Germany. Shizuka.Uchida@mpi-bn.mpg.de
Identifiers:ISSN:1471-2164 (Electronic) 1471-2164 (Linking)
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=..
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