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          Institute: MPI für Herz- und Lungenforschung (W. G. Kerckhoff Institut)     Collection: Publikationen des W. G. Kerckhoff-Instituts     Display Documents

ID: 474467.0, MPI für Herz- und Lungenforschung (W. G. Kerckhoff Institut) / Publikationen des W. G. Kerckhoff-Instituts
Evaluation of angiogenesis using micro-computed tomography in a xenograft mouse model of lung cancer
Authors:Savai, R.; Langheinrich, A. C.; Schermuly, R. T.; Pullamsetti, S. S.; Dumitrascu, R.; Traupe, H.; Rau, W. S.; Seeger, W.; Grimminger, F.; Banat, G. A.
Date of Publication (YYYY-MM-DD):2009
Title of Journal:Neoplasia
Issue / Number:1
Start Page:48
End Page:56
Audience:Not Specified
Abstract / Description:Quantitative evaluation of lung tumor angiogenesis using immunohistochemical techniques has been limited by difficulties in generating reproducible data. To analyze intrapulmonary tumor angiogenesis, we used high-resolution micro-computed tomography (micro-CT) of lung tumors of mice inoculated with mouse Lewis lung carcinoma (LLC1) or human adenocarcinoma (A549) cell lines. The lung vasculature was filled with the radiopaque silicone rubber, Microfil, through the jugular vein (in vivo application) or pulmonary artery (ex vivo application). In addition, human adenocarcinoma lung tumor-bearing mice treated site-specifically with humanized monoclonal antibody (bevacizumab) against vascular endothelial growth factor. Quantitative analysis of lung tumor microvessels imaged with micro-CT showed that more vessels (mainly small, <0.02 mm(2)) were filled using the in vivo (5.4%) compared with the ex vivo (2.1%) method. Furthermore, bevacizumab-treated lung tumor-bearing mice showed significantly reduced lung tumor volume and lung tumor angiogenesis compared with untreated mice as assessed by micro-CT. Interestingly, microvascularization of mainly the smaller vessels (<0.02 mm(2)) was reduced after bevacizumab treatment. This observation with micro-CT was nicely correlated with immunohistochemical measurement of microvessels. Therefore, micro-CT is a novel method for investigating lung tumor angiogenesis, and this might be considered as an additional complementary tool for precise quantification of angiogenesis.
Free Keywords:Angiogenesis Inhibitors/administration & dosage; Animals; Antibodies, Monoclonal/administration & dosage; Carcinoma/pathology/*radiography; Drug Delivery Systems; Feasibility Studies; Female; Humans; Image Interpretation, Computer-Assisted; Lung Neoplasms/blood supply/drug therapy/pathology/*radiography; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Mice, Nude; Neovascularization, Pathologic/drug therapy/*radiography; Organ Specificity/drug effects; Tumor Cells, Cultured; X-Ray Microtomography/*methods; Xenograft Model Antitumor Assays
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:MPI für physiologische und klinische Forschung
External Affiliations:Department of Hematology and Oncology, Justus-Liebig-University, Giessen, Germany. Savai.Rajkumar@innere.med.uni-giessen.de
Identifiers:ISSN:1476-5586 (Electronic) 1476-5586 (Linking)
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