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          Institute: MPI für Herz- und Lungenforschung (W. G. Kerckhoff Institut)     Collection: Publikationen des W. G. Kerckhoff-Instituts     Display Documents

ID: 474494.0, MPI für Herz- und Lungenforschung (W. G. Kerckhoff Institut) / Publikationen des W. G. Kerckhoff-Instituts
Stem cell-mediated natural tissue engineering
Authors:Mollmann, H.; Nef, H. M.; Voss, S.; Troidl, C.; Willmer, M.; Szardien, S.; Rolf, A.; Klement, M.; Voswinckel, R.; Kostin, S.; Ghofrani, H. A.; Hamm, C. W.; Elsasser, A.
Date of Publication (YYYY-MM-DD):2009
Title of Journal:J Cell Mol Med
Audience:Not Specified
Abstract / Description:ABSTRACT Background-Recently, we demonstrated that a fully differentiated tissue developed on a ventricular septal occluder that had been implanted due to infarct-related septum rupture. We hypothesized that this tissue originated from circulating stem cells. The aim of the present study was to evaluate this hypothesis and to investigate the physiological differentiation and transdifferentiation potential of circulating stem cells. Methods and Results-We developed an animal model in which a freely floating membrane was inserted into each the left ventricle and the descending aorta. Membranes were removed after prespecified intervals of 3 days, and 2, 6, and 12 weeks; the newly developed tissue was evaluated using quantitative RT-PCR, immunohistochemistry, and in situ hybridization. The contribution of stem cells was directly evaluated in another group of animals that were by treated with GM-CSF early after implantation. We demonstrated the time-dependent generation of a fully differentiated tissue composed of fibroblasts, myofibroblasts, smooth muscle cells, endothelial cells, and new blood vessels. Cells differentiated into early cardiomyocytes on membranes implanted in the left ventricles but not on those implanted in the aortas. Stem cell mobilization with GM-CSF led to more rapid tissue growth and differentiation. The GM-CSF effect on cell proliferation outlasted the treatment period by several weeks. Conclusions-Circulating stem cells contributed to the development of a fully differentiated tissue on membranes placed within the left ventricle or descending aorta under physiological conditions. Early cardiomyocyte generation was identified only on membranes positioned within the left ventricle.
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:MPI für physiologische und klinische Forschung
External Affiliations:Kerckhoff Heart Center, Dept. of Cardiology, 61231 Bad Nauheim, Germany.
Identifiers:ISSN:1582-4934 (Electronic) 1582-1838 (Linking)
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