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          Institute: MPI für Herz- und Lungenforschung (W. G. Kerckhoff Institut)     Collection: Publikationen des W. G. Kerckhoff-Instituts     Display Documents

ID: 474513.0, MPI für Herz- und Lungenforschung (W. G. Kerckhoff Institut) / Publikationen des W. G. Kerckhoff-Instituts
The proteoglycan osteoglycin/mimecan is correlated with arteriogenesis
Authors:Kampmann, A.; Fernandez, B.; Deindl, E.; Kubin, T.; Pipp, F.; Eitenmuller, I.; Hoefer, I. E.; Schaper, W.; Zimmermann, R.
Date of Publication (YYYY-MM-DD):2009
Title of Journal:Mol Cell Biochem
Issue / Number:1-2
Start Page:15
End Page:23
Audience:Not Specified
Abstract / Description:Arteriogenesis or collateral growth is able to compensate for the stenosis of major arteries. Using differential display RT-PCR on growing and quiescent collateral arteries in a rabbit femoral artery ligation model, we cloned the rabbit full-length cDNA of osteoglycin/mimecan. Osteoglycin was present in the adventitia of collateral arteries as a glycosylated protein without keratan sulfate side chains, mainly produced by smooth muscle cells (SMCs) and perivascular fibroblasts. Northern blot, Western blot, and immunohistochemistry confirmed a collateral artery-specific downregulation of osteoglycin from 6 h to 3 weeks after the onset of arteriogenesis. Treatment of primary SMCs with the arteriogenic protein fibroblast growth factor-2 (FGF-2) resulted in a similar reduction of osteoglycin expression as observed in vivo. Application of the FGF-2 inhibitor polyanethole sulfonic acid (PAS) blocked the downregulation of osteoglycin and interfered with arteriogenesis. From our study we conclude that downregulation of osteoglycin is a fundamental requirement for proper arteriogenesis.
Free Keywords:Amino Acid Sequence; Animals; Arteries/growth & development; Base Sequence; DNA, Complementary/chemistry; Down-Regulation; Femoral Artery/*growth & development; Fibroblast Growth Factor 2/metabolism; Models, Animal; Molecular Sequence Data; Muscle, Smooth, Vascular/metabolism; Proteoglycans/chemistry/genetics/*metabolism; RNA, Messenger/metabolism; Rabbits; Reverse Transcriptase Polymerase Chain Reaction
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:MPI für physiologische und klinische Forschung
External Affiliations:Research Group Vascular Genomics, Kerckhoff Clinic, Bad Nauheim, Germany.
Identifiers:ISSN:1573-4919 (Electronic) 0300-8177 (Linking)
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