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          Institute: MPI für Polymerforschung     Collection: MPI Polymerforschung     Display Documents



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ID: 48895.0, MPI für Polymerforschung / MPI Polymerforschung
Preparation of Self-Assembled Monolayers Containing Anthryl Groups toward Hybridization of Nucleotides
Authors:Nakamura, F.; Mitsui, K.; Hara, M.; Kraemer, S.; Mittler, Silvia; Knoll, Wolfgang
Language:English
Date of Publication (YYYY-MM-DD):2003
Title of Journal:Langmuir
Volume:19
Issue / Number:14
Start Page:5823
End Page:5829
Review Status:not specified
Audience:Not Specified
Abstract / Description:To realize immobilization and hybridization of nucleotides, self-assembled monolayers (SAMs) containing anthryl groups which interact with nucleotides were prepared on a gold substrate. We used an unsymmetric disulfide containing an anthryl group (Anth-s-s-C8), which can form a SAM on a gold substrate, to immobilize nucleotides on the gold surface via the intercalation between DNA and anthryl groups. The SAM was characterized by time-of-flight mass spectroscopy. The interaction between polynucleotides and the SAM and hybridization were investigated by surface plasmon resonance. The surface coverage of anthryl groups on the SAM could be controlled by mixing Anth-s-s-C8 and 11-mercapto-1-undecanol in solution at various ratios. The efficient hybridization of polynucleotides could be realized by controlling 30% of Anth-s-s-C8 on the surface. This method can be applied to oligonucleotides, and the efficiency of hybridization is enhanced by introducing the double-stranded portion in the middle.
External Publication Status:published
Document Type:Article
Affiliations:MPI für Polymerforschung
External Affiliations:RIKEN, Inst Phys & Chem Res, Frontier Res Syst, Wako, Saitama 3510198, Japan; Japan Sci & Technol, PRESTO, JST, Kawaguchi, Saitama 3350012, Japan
Identifiers:LOCALID:P-03-56
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