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          Institute: MPI für Neurobiologie     Collection: Neuroimmunology     Display Documents



  history
ID: 508165.0, MPI für Neurobiologie / Neuroimmunology
T cell vaccination induces the elimination of EAE effector T cells: Analysis using GFP-transduced, encephalitogenic T cells
Authors:Volovitz, I.; Marmor, Y.; Mor, F.; Fluegel, A.; Odoardi, F.; Eisenbach, L.; Cohen, I. R.
Language:English
Date of Publication (YYYY-MM-DD):2010-09
Title of Journal:Journal of Autoimmunity
Journal Abbrev.:J. Autoimmun.
Volume:35
Issue / Number:2
Start Page:135
End Page:144
Review Status:Peer-review
Audience:Not Specified
Abstract / Description:T cell vaccination (TCV) with irradiated encephalitogenic T cells induces resistance to EAE. However, the fate of the encephalitogenic T cells in vivo following TCV has yet to be studied. Here we used anti-MBP encephalitogenic T cells that were transduced to express GFP to study the effects of TCV on these cells. In naive rats or in control-vaccinated (Ova-GFP) rats injected i.v. with GFP-labeled effector cells, high numbers of effector T cells were found along with macrophages, CD8 T cells and Non-GFP CD4 cells in the spleens, parathymic lymph nodes (PTLN) and spinal cords. In contrast, the recipients that had been treated with TCV (anti-MBP T-cell lines) showed few if any GFP-labeled effector T cells throughout the disease (day 1-8) and their spinal cords were almost clear of macrophages, CD4 and CD8 cells. Splenocytes in the control groups secreted IFN gamma in response to MBP and showed high numbers of IFN gamma secreting CD4 and CD8 cells in their spinal cords at the disease peak. In the TCV-protected groups, splenocytes showed no reactivity to MBP but secreted IFN gamma in response to irradiated encephalitogenic T cells - an anti-idiotypic response. Thus. TCV leads to a marked decrease in the numbers of effector T cells in the CNS and lymphoid organs, to a marked reduction in the Th1 cytokine producing cells in the CNS, and to the appearance of T cells responsive to the anti-MBP effector T cells. (C) 2010 Elsevier Ltd. All rights reserved.
Free Keywords:Rodent - rat; Experimental autoimmune; encephalomyelitis - EAE; Multiple sclerosis - MS; T cell vaccination - TCV; T cells; Cell tracking methods
External Publication Status:published
Document Type:Article
Affiliations:MPI für Neurobiologie/Neuroimmunology (Wekerle)
External Affiliations:[Volovitz, Ilan; Marmor, Yotvat; Mor, Felix; Eisenbach, Lea; Cohen, Irun R.] Weizmann Inst Sci, Dept Immunol, IL-76100 Rehovot, Israel.; [Volovitz, Ilan] Tel Aviv Sourasky Med Ctr, Dept Neurosurg, IL-64239 Tel Aviv, Israel.
Identifiers:ISI:000281986100005 [ID No:1]
ISSN:0896-8411 [ID No:2]
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