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          Institute: MPI für Informatik     Collection: Computational Biology and Applied Algorithmics     Display Documents



ID: 520907.0, MPI für Informatik / Computational Biology and Applied Algorithmics
Impact of a single amino acid in the variable region 2 of the Old World monkey TRIM5alpha SPRY ({B}30.2) domain on anti-human immunodeficiency virus type 2 activity
Authors:Kono, Ken; Bozek, Katarzyna; Domingues, Francisco S.; Shioda, Tatsuo; Nakayama, Emi E.
Language:English
Date of Publication (YYYY-MM-DD):2009
Title of Journal:Virology
Volume:388
Issue / Number:1
Start Page:160
End Page:168
Review Status:Peer-review
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:Variable region 1 (V1) of the SPRY domain of TRIM5alpha is a major determinant
for species-specific virus restriction in primates. We previously reported that
a chimeric TRIM5alpha containing baboon V1 in the background of cynomolgus
monkey TRIM5alpha showed potent anti-human immunodeficiency virus type 2
(HIV-2) activity. Since baboons are reportedly sensitive to HIV-2 infection,
there was a discrepancy between the ability of baboon TRIM5alpha V1 to restrict
HIV-2 and baboon sensitivity to HIV-2. In the study presented here, we examined
the roles of V2 and V3 of the baboon TRIM5alpha SPRY domain in its anti-HIV-2
activity. A chimeric TRIM5alpha containing the entire baboon SPRY domain showed
weak anti-HIV-2 activity. This attenuation of activity was caused by a single
serine-to-proline substitution in baboon TRIM5alpha V2. These findings indicate
that the combination of V1 with other variable regions of SPRY is important in
anti-HIV-2 activity of primate TRIM5alpha.
Last Change of the Resource (YYYY-MM-DD):2009-12-21
External Publication Status:published
Document Type:Article
Communicated by:Thomas Lengauer
Affiliations:MPI für Informatik/Computational Biology and Applied Algorithmics
Identifiers:LOCALID:C125673F004B2D7B-4DD2923739BBD81BC12576930044E44D-...
URL:http://dx.doi.org/10.1016/j.virol.2009.03.004
DOI:10.1016/j.virol.2009.03.004
ISSN:0042-6822
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