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          Institute: MPI für Neurobiologie     Collection: Systems and Computational Neurobiology     Display Documents



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ID: 527658.0, MPI für Neurobiologie / Systems and Computational Neurobiology
Neurons with Cholinergic Phenotype in the Visual System of Drosophila
Authors:Raghu, S. V.; Reiff, D. F.; Borst, A.
Language:English
Date of Publication (YYYY-MM-DD):2011-01-01
Title of Journal:Journal of Comparative Neurology
Journal Abbrev.:J. Comp. Neurol.
Volume:519
Issue / Number:1
Start Page:162
End Page:176
Review Status:Peer-review
Audience:Not Specified
Abstract / Description:The optic lobe of Drosophila houses about 60,000 neurons that are organized in parallel, retinotopically arranged columns. Based on the Golgi-staining method, Fischbach and Dittrich ([1989] Cell Tissue Res 258:441-475) determined that each column contains about 90 identified cells. Each of these cells is supposed to release one or two different neurotransmitters. However, for most cells the released neurotransmitter is not known. Here we characterize the vast majority of the neurons in the Drosophila optic lobe that release acetylcholine (Ach), the major excitatory neurotransmitter of the insect central nervous system. We employed a promoter specific for cholinergic neurons and restricted its activity to single or a few cells using the MARCM technique. This approach allowed us to establish an anatomical map of neurons with a cholinergic phenotype based on their branching pattern. We identified 43 different types of neurons with a cholinergic phenotype. Thirty-one of them match previously described members of nine different subgroups: Transmedullary (Tm), Transmedullary Y (TmY), Medulla intrinsic (Mi, Mt, and Pm), Bushy T (T), Translobula Plate (Tlp), and Lobula intrinsic (Lcn and Lt) neurons (Fischbach and Dittrich [1989]). Intriguingly, 12 newly identified cell types suggest that previous Golgi studies were not saturating and that the actual number of different neurons per column is higher than previously thought. This study and similar ones on other neurotransmitter systems will contribute towards a columnar wiring diagram and foster the functional dissection of the visual circuitry in Drosophila. J. Comp. Neurol. 519:162-176, 2011. (C) 2010 Wiley-Liss, Inc.
Free Keywords:Drosophila; cholinergic; Cha; MARCM; visual system; insects
External Publication Status:published
Document Type:Article
Version Comment:Automatic journal name synchronization
Affiliations:MPI für Neurobiologie/Systems and Computational Neurobiology (Borst)
Identifiers:ISI:000285310100011 [ID No:1]
ISSN:0021-9967 [ID No:2]
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