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          Institute: MPI für Infektionsbiologie     Collection: RNA Biology     Display Documents



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ID: 528363.0, MPI für Infektionsbiologie / RNA Biology
Evidence for an autonomous 5 ' target recognition domain in an Hfq-associated small RNA
Authors:Papenfort, Kai; Bouvier, Marie; Mika, Franziska; Sharma, Cynthia Mira; Vogel, Jörg
Language:English
Date of Publication (YYYY-MM-DD):2010-11-23
Title of Journal:Proceedings of the National Academy of Sciences of the United States of America
Journal Abbrev.:Proc. Natl. Acad. Sci. U. S. A.
Volume:107
Issue / Number:47
Start Page:20435
End Page:20440
Copyright:Copyright ©2011 by the National Academy of Sciences
Freely available online through the PNAS open access option.
Review Status:Peer-review
Audience:Experts Only
Abstract / Description:The abundant class of bacterial Hfq-associated small regulatory RNAs (sRNAs) parallels animal microRNAs in their ability to control multiple genes at the posttranscriptional level by short and imperfect base pairing. In contrast to the universal length and seed pairing mechanism of microRNAs, the sRNAs are heterogeneous in size and structure, and how they regulate multiple targets is not well understood. This paper provides evidence that a 5' located sRNA domain is a critical element for the control of a large posttranscriptional regulon. We show that the conserved 5' end of RybB sRNA recognizes multiple mRNAs of Salmonella outer membrane proteins by >= 7-bp Watson-Crick pairing. When fused to an unrelated sRNA, the 5' domain is sufficient to guide target mRNA degradation and maintain sigma(E)-dependent envelope homeostasis. RybB sites in mRNAs are often conserved and flanked by 3' adenosine. They are found in a wide sequence window ranging from the upstream untranslated region to the deep coding sequence, indicating that some targets might be repressed at the level of translation, whereas others are repressed primarily by mRNA destabilization. Autonomous 5' domains seem more common in sRNAs than appreciated and might improve the design of synthetic RNA regulators.
Free Keywords:envelope stress; multiple targeting; noncoding RNA; porin
Comment of the Author/Creator:Acknowledgments
We thank F. Seifert for technical assistance, R. Misra (Tempe, AZ) and D. Linke (Tübingen, Germany) for porin antisera, V. Pfeiffer for plasmid construction, F. Darfeuille (Université de Bordeaux, France) for LNA synthesis, This work was supported by a European Molecular Biology Organization long-term fellowship (to M.B.) and by funds from the Deutsche Forschungsgemeinschaft Priority Program SPP1258 Sensory and Regulatory RNAs in Prokaryotes (to J.V.).
External Publication Status:published
Document Type:Article
Version Comment:Automatic journal name synchronization
Communicated by:Hilmar Fünning
Affiliations:MPI für Infektionsbiologie/RNA Biology
External Affiliations:Univ Wurzburg, Inst Mol Infect Biol, D-97080 Wurzburg, Germany.
Identifiers:ISI:000284529000054 [ID No:1]
ISSN:0027-8424 [ID No:2]
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