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          Institute: MPI für Biophysik     Collection: Abt. Molekulare Membranbiologie     Display Documents

ID: 530618.0, MPI für Biophysik / Abt. Molekulare Membranbiologie
Functional Modules and Structural Basis of Conformational Coupling in Mitochondrial Complex I
Authors:Hunte, Carola; Zickermann, Volker; Brandt, Ulrich
Date of Publication (YYYY-MM-DD):2010-07-23
Title of Journal:Science
Start Page:448
End Page:451
Review Status:Peer-review
Audience:Experts Only
Abstract / Description:Proton-pumping respiratory complex I is one of the largest and most complicated membrane protein complexes. Its function is critical for efficient energy supply in aerobic cells, and malfunctions are implicated in many neurodegenerative disorders. Here, we report an x-ray crystallographic analysis of mitochondrial complex I. The positions of all iron-sulfur clusters relative to the membrane arm were determined in the complete enzyme complex. The ubiquinone reduction site resides close to 30 angstroms above the membrane domain. The arrangement of functional modules suggests conformational coupling of redox chemistry with proton pumping and essentially excludes direct mechanisms. We suggest that a ~60-angstrom-long helical transmission element is critical for transducing conformational energy to proton-pumping elements in the distal module of the membrane arm.
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:MPI für Biophysik/Abteilung Molekulare Membranbiologie
External Affiliations:Institute for Biochemistry and Molecular Biology, Centre for Biological Signalling Studies (BIOSS), University of Freiburg, D-79104 Freiburg, Germany;
Institute of Membrane and Systems Biology, University of Leeds, Leeds LS2 9JT, UK;
Molecular Bioenergetics Group, Medical School, Cluster of Excellence, Frankfurt “Macromolecular Complexes,” Center for Membrane Proteomics, Goethe-University, Frankfurt am Main, Germany
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