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          Institute: MPI für Biophysik     Collection: Abt. Biophysikalische Chemie     Display Documents



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ID: 534143.0, MPI für Biophysik / Abt. Biophysikalische Chemie
Light induced stimulation and delay of cardiac activity
Authors:Hofmann, Boris; Maybeck, Vanessa; Eick, Stefan; Meffert, Simone; Ingebrandt, Sven; Wood, Philip; Bamberg, Ernst; Offenhäusser, Andreas
Language:English
Date of Publication (YYYY-MM-DD):2010
Title of Journal:Lab on a Chip
Journal Abbrev.:Lab Chip
Volume:10
Issue / Number:19
Start Page:2588
End Page:2596
Review Status:Peer-review
Audience:Experts Only
Abstract / Description:This article shows the combination of light activatable ion channels and microelectrode array (MEA) technology for bidirectionally interfacing cells. HL-1 cultures, a mouse derived cardiomyocyte-like cell line, transfected with channelrhodopsin were stimulated with a microscope coupled 473 nm laser and recorded with custom built 64 electrode MEAs. Channelrhodopsin induced depolarization of the cell can evoke action potentials (APs) in single cells. Spreading of the AP over the cell layer can then be measured with good spatiotemporal resolution using MEA recordings. The possibility for light induced pacemaker switching in cultures was shown. Furthermore, the suppression of APs can also be achieved with the laser. Possible applications include cell analysis, e.g. pacemaker interference or induced pacemaker switching, and medical applications such as a combined cardiac pacemaker and defibrillator triggered by light. Since current prosthesis research focuses on bidirectionality, this system may be applied to any electrogenic cell, including neurons or muscles, to advance this field.
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:MPI für Biophysik/Abteilung Biophysikalische Chemie
External Affiliations:Institute of Bio- and Nanosystems—Bioelectronics (IBN-2) and Jara-FIT, Forschungszentrum Jülich, Leo-Brandt-Str., D-52425 Jülich, Germany;
Department of Informatics and Microsystem Technology, University of Applied Sciences Kaiserslautern, D-66482 Zweibrücken, Germany;
Institut für Biophysikalische Chemie, Johann Wolfgang Goethe University, Max-von-Laue-Strasse 9, D-60438 Frankfurt, Germany
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