MPI für molekulare Genetik / Sequencing Group |
|BISMA--fast and accurate bisulfite sequencing data analysis of individual clones from unique and repetitive sequences|
|Authors:||Rohde, Christian; Zhang, Yingying; Reinhardt, Richard; Jeltsch, Albert|
|Date of Publication (YYYY-MM-DD):||2010-05-06|
|Title of Journal:||BMC Bioinformatics|
|Copyright:||© 2010 Rohde et al; licensee BioMed Central Ltd|
|Review Status:||not specified|
|Abstract / Description:||Background
Bisulfite sequencing is a popular method to analyze DNA methylation patterns at high resolution. A region of interest is targeted by PCR and about 20-50 subcloned DNA molecules are usually analyzed, to determine the methylation status at single CpG sites and molecule resolution.
The BISMA (Bisulfite Sequencing DNA Methylation Analysis) software for analysis of primary bisulfite sequencing data implements sequencing data extraction and enhanced data processing, quality controls, analysis and presentation of the methylation state. It uses an improved strategy for detection of clonal molecules and accurate CpG site detection and it supports for the first time analysis of repetitive sequences.
BISMA works highly automated but still provides the user full control over all steps of the analysis. The BISMA software is freely available as an online tool for academic purposes for the analysis of bisulfite sequencing data from both unique and repetitive sequences http://biochem.jacobs-university.de/BDPC/BISMA/ webcite.
|Comment of the Author/Creator:||This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.|
|External Publication Status:||published|
|Communicated by:||Richard Reinhardt|
|Affiliations:||MPI für molekulare Genetik|
|External Affiliations:||School of Engineering and Science, Jacobs University Bremen, Campus Ring 1, 28725 Bremen, Germany|