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| ID:
536146.0,
MPI für molekulare Genetik / Sequencing Group |
| Cultivation-independent characterization of ‘Candidatus Magnetobacterium bavaricum’ via ultrastructural, geochemical, ecological and metagenomic methods |
| Authors: | Jogler, C.; Niebler, M.; Lin, W.; Kube, M.; Wanner, G.; Kolinko, S.; Stief, P.; Beck, A. J.; De Beer, D.; Petersen, N.; Pan, Y.; Amann, R.; Reinhardt, R.; Schüler, D. | | Language: | English | | Date of Publication (YYYY-MM-DD): | 2010-09 | | Title of Journal: | Environmental Microbiology | | Journal Abbrev.: | Environ Microbiol | | Volume: | 12 | | Issue / Number: | 9 | | Start Page: | 2466 | | End Page: | 2478 | | Copyright: | © 2010 Society for Applied Microbiology and Blackwell Publishing Ltd | | Review Status: | not specified | | Audience: | Experts Only | | Abstract / Description: | ‘Candidatus Magnetobacterium bavaricum’ is unusual among magnetotactic bacteria (MTB) in terms of cell size (8–10 µm long, 1.5–2 µm in diameter), cell architecture, magnetotactic behaviour and its distinct phylogenetic position in the deep-branching Nitrospira phylum. In the present study, improved magnetic enrichment techniques permitted high-resolution scanning electron microscopy and energy dispersive X-ray analysis, which revealed the intracellular organization of the magnetosome chains. Sulfur globule accumulation in the cytoplasm point towards a sulfur-oxidizing metabolism of ‘Candidatus M. bavaricum’. Detailed analysis of ‘Candidatus M. bavaricum’ microhabitats revealed more complex distribution patterns than previously reported, with cells predominantly found in low oxygen concentration. No correlation to other geochemical parameters could be observed. In addition, the analysis of a metagenomic fosmid library revealed a 34 kb genomic fragment, which contains 33 genes, among them the complete rRNA gene operon of ‘Candidatus M. bavaricum’ as well as a gene encoding a putative type IV RubisCO large subunit. | | External Publication Status: | published | | Document Type: | Article |
| Communicated by: | Richard Reinhardt | | Affiliations: | MPI für molekulare Genetik
| | External Affiliations: | LMU Department Biology I, Microbiology,
Großharderner-Str. 2, 82152, Planegg-Martinsried,
Germany
Biogeomagnetism Group, Paleomagnetism and
Geochronology Laboratory, State Key Laboratory of
Lithospheric Evolution, Institute of Geology and
Geophysics, Chinese Academy of Sciences, Beijing
100029, China
Max Planck Institute for Marine Microbiology,
Celsiusstr. 1, 28359 Bremen, Germany
LMU Department of Earth and Environmental Sciences,
Geophysics, Theresienstr. 41, 80333 Munich Germany
| | Identifiers: | DOI:10.1111/j.1462-2920.2010.02220.x
ISSN:1462-2912
URL:http://onlinelibrary.wiley.com/doi/10.1111/j.1462-...
URL:http://onlinelibrary.wiley.com/store/10.1111/j.146... [Supporting information] | |
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