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          Institute: MPI für molekulare Genetik     Collection: Sequencing Group     Display Documents



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ID: 536222.0, MPI für molekulare Genetik / Sequencing Group
Deep sequencing-based discovery of the Chlamydia trachomatis transcriptome
Authors:Albrecht, Marco; Sharma, Cynthia Mira; Reinhardt, Richard; Vogel, Jörg; Rudel, Thomas
Language:English
Date of Publication (YYYY-MM-DD):2010-01
Title of Journal:Nucleic Acids Research
Journal Abbrev.:Nucleic Acids Res
Volume:38
Issue / Number:3
Start Page:868
End Page:877
Copyright:© The Author(s) 2010. Published by Oxford University Press.
Review Status:Peer-review
Audience:Experts Only
Abstract / Description:Chlamydia trachomatis is an obligate intracellular pathogenic bacterium that has been refractory to genetic manipulations. Although the genomes of several strains have been sequenced, very little information is available on the gene structure of these bacteria. We used deep sequencing to define the transcriptome of purified elementary bodies (EB) and reticulate bodies (RB) of C. trachomatis L2b, respectively. Using an RNA-seq approach, we have mapped 363 transcriptional start sites (TSS) of annotated genes. Semi-quantitative analysis of mapped cDNA reads revealed differences in the RNA levels of 84 genes isolated from EB and RB, respectively. We have identified and in part confirmed 42 genome- and 1 plasmid-derived novel non-coding RNAs. The genome encoded non-coding RNA, ctrR0332 was one of the most abundantly and differentially expressed RNA in EB and RB, implying an important role in the developmental cycle of C. trachomatis. The detailed map of TSS in a thus far unprecedented resolution as a complement to the genome sequence will help to understand the organization, control and function of genes of this important pathogen.
Comment of the Author/Creator:This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
External Publication Status:published
Document Type:Article
Communicated by:Richard Reinhardt
Affiliations:MPI für molekulare Genetik
MPI für Infektionsbiologie/RNA Biology
External Affiliations:Univ Wurzburg, Dept Microbiol, Bioctr, D-97074 Wurzburg, Germany
Identifiers:ISI:000274496900021 [ID No:1]
ISSN:0305-1048 [ID No:2]
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