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          Institute: MPI für molekulare Genetik     Collection: Department of Vertebrate Genomics     Display Documents

ID: 541905.0, MPI für molekulare Genetik / Department of Vertebrate Genomics
Cryo-EM structure and rRNA model of a translating eukaryotic 80S ribosome at 5.5-A resolution
Authors:Armache, J. P.; Jarasch, A.; Anger, A. M.; Villa, E.; Becker, T.; Bhushan, S.; Jossinet, F.; Habeck, M.; Dindar, G.; Franckenberg, S.; Marquez, V.; Mielke, T.; Thomm, M.; Berninghausen, O.; Beatrix, B.; Soding, J.; Westhof, E.; Wilson, D. N.; Beckmann, R.
Date of Publication (YYYY-MM-DD):2010-11-16
Title of Journal:Preceedings of the National Academy of Sciences USA
Journal Abbrev.:Proc Natl Acad Sci USA
Issue / Number:46
Start Page:19748
End Page:19753
Copyright:© 2010 by the National Academy of Sciences
Review Status:not specified
Audience:Experts Only
Abstract / Description:Protein biosynthesis, the translation of the genetic code into polypeptides, occurs on ribonucleoprotein particles called ribosomes. Although X-ray structures of bacterial ribosomes are available, high-resolution structures of eukaryotic 80S ribosomes are lacking. Using cryoelectron microscopy and single-particle reconstruction, we have determined the structure of a translating plant (Triticum aestivum) 80S ribosome at 5.5-A resolution. This map, together with a 6.1-A map of a Saccharomyces cerevisiae 80S ribosome, has enabled us to model approximately 98% of the rRNA. Accurate assignment of the rRNA expansion segments (ES) and variable regions has revealed unique ES-ES and r-protein-ES interactions, providing insight into the structure and evolution of the eukaryotic ribosome.
Free Keywords:Cryoelectron Microscopy; Crystallography, X-Ray; Escherichia coli/metabolism/ultrastructure; Eukaryotic Cells/metabolism/ultrastructure; Humans; Models, Molecular; Protein Biosynthesis; RNA, Ribosomal/ultrastructure; Ribosomes/chemistry/metabolism/ultrastructure; Saccharomyces cerevisiae/metabolism/ultrastructure; Triticum/metabolism/ultrastructure
External Publication Status:published
Document Type:Article
Communicated by:Hans Lehrach
Affiliations:MPI für molekulare Genetik
MPI für Biochemie
MPI für biologische Kybernetik
MPI für Entwicklungsbiologie
External Affiliations:Gene Center and Center for Integrated Protein Science Munich, Department of Biochemistry, Ludwig-Maximilians-Universität München, Feodor-Lynen-Strasse 25, 81377 Munich, Germany
University de Strasbourg, Institut de Biologie Moleculaire et Cellulaire du Centre National de la Recherche Scientifique, 15 Rue René Descartes, 67084 Strasbourg, France
Institut für Medizinische Physik und Biophysik, Charité, Ziegelstrasse 5-8, 10117 Berlin, Germany
Universität Regensburg, Lehrstuhl für Mikrobiologie, Universitätstrasse 31, 93053 Regensburg, Germany
Identifiers:ISSN:1091-6490 (Electronic) [ID No:1]
DOI:10.1073/pnas.1009999107 [ID No:2]
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