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          Institute: MPI für molekulare Genetik     Collection: Department of Vertebrate Genomics     Display Documents



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ID: 541908.0, MPI für molekulare Genetik / Department of Vertebrate Genomics
Localization of eukaryote-specific ribosomal proteins in a 5.5-A cryo-EM map of the 80S eukaryotic ribosome
Authors:Armache, J. P.; Jarasch, A.; Anger, A. M.; Villa, E.; Becker, T.; Bhushan, S.; Jossinet, F.; Habeck, M.; Dindar, G.; Franckenberg, S.; Marquez, V.; Mielke, T.; Thomm, M.; Berninghausen, O.; Beatrix, B.; Soding, J.; Westhof, E.; Wilson, D. N.; Beckmann, R.
Language:English
Date of Publication (YYYY-MM-DD):2010-11-16
Title of Journal:Proceedings of the National Academy of Sciences USA
Journal Abbrev.:Proc Natl Acad Sci USA
Volume:107
Issue / Number:46
Start Page:19754
End Page:19759
Copyright:© 2010 by the National Academy of Sciences
Review Status:not specified
Audience:Experts Only
Abstract / Description:Protein synthesis in all living organisms occurs on ribonucleoprotein particles, called ribosomes. Despite the universality of this process, eukaryotic ribosomes are significantly larger in size than their bacterial counterparts due in part to the presence of 80 r proteins rather than 54 in bacteria. Using cryoelectron microscopy reconstructions of a translating plant (Triticum aestivum) 80S ribosome at 5.5-A resolution, together with a 6.1-A map of a translating Saccharomyces cerevisiae 80S ribosome, we have localized and modeled 74/80 (92.5%) of the ribosomal proteins, encompassing 12 archaeal/eukaryote-specific small subunit proteins as well as the complete complement of the ribosomal proteins of the eukaryotic large subunit. Near-complete atomic models of the 80S ribosome provide insights into the structure, function, and evolution of the eukaryotic translational apparatus.
Free Keywords:Cryoelectron Microscopy; Eukaryotic Cells/metabolism/ultrastructure; Evolution, Molecular; Models, Molecular; Protein Transport; RNA, Ribosomal/chemistry/genetics/ultrastructure; Ribosomal Proteins/metabolism/ultrastructure; Ribosomes/metabolism/ultrastructure; Saccharomyces cerevisiae/metabolism/ultrastructure; Species Specificity; Triticum/metabolism
External Publication Status:published
Document Type:Article
Communicated by:Hans Lehrach
Affiliations:MPI für molekulare Genetik
MPI für Biochemie
MPI für biologische Kybernetik
MPI für Entwicklungsbiologie
External Affiliations:Gene Center and Center for integrated Protein Science Munich, Department of Biochemistry, Ludwig-Maximilians-Universität München, Feodor-Lynen-Strasse 25, 81377 Munich, Germany
Université de Strasbourg, Institut de Biologie Moléculaire et Cellulaire du Centre National de la Recherche Scientifique, 15 Rue René Descartes, 67084 Strasbourg, France
Institut für Medizinische Physik und Biophysik, Charité, Ziegelstrasse 5-8, 10117 Berlin, Germany
Universität Regensburg, Lehrstuhl für Mikrobiologie, Universitätstrasse 31, 93053 Regensburg, Germany
Identifiers:ISSN:1091-6490 (Electronic) [ID No:1]
DOI:10.1073/pnas.1010005107 [ID No:2]
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