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          Institute: MPI für Physik     Collection: MPI für Physik     Display Documents



ID: 543271.0, MPI für Physik / MPI für Physik
High detection sensitivity achieved with cryogenic detectors in combination with matrix-assisted laser desorption / ionisation time-of-flight mass spectrometry (MALDI-TOF MS)
Authors:Christ, P.; Rutzinger, S.; Koy, C.; Seidel, W.; Uchaikin, S.; Glocker, M. O.; Pröbst, F.
Date of Publication (YYYY-MM-DD):2004
Title of Journal:European Journal of Mass Spectrometry
Journal Abbrev.:Eur.J.Mass Spectrom.
Issue / Number:10
Start Page:469
End Page:476
Audience:Not Specified
Intended Educational Use:No
Abstract / Description:Cryogenic detectors directly measure the impact energy of any impinging particle independent of its velocity. Thus a very high, mass-independent, detection efficiency is expected from their application in TOF-MS. The cryogenic detector applied here is based on a superconducting phase-transition thermometer and was implemented in a dual reflector time-of-flight mass spectrometer (N-geometry). A dilution series using standard sample preparation procedures shows that the detection limit for insulin (Mr: 5,734) can be decreased by several orders of magnitude, down to 0.5 amol on the MALDI target. Detection limits for rhM-CSF ß (Mr: 49,032) and for polyclonal IgG (Mr: ca. 150,000) in the high femtomole and low picomole range, respectively, were established.
Classification / Thesaurus:Experimental Physics
Comment of the Author/Creator:submitted to European Journal of Mass Spectrometry
accepted on 6th May 2004
External Publication Status:published
Document Type:Article
Communicated by:N.N.
Affiliations:MPI für Physik
Identifiers:LOCALID:MPP-2003-141
URL:http://publications.mppmu.mpg.de/?action=search&mp...
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