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          Institute: MPI für medizinische Forschung     Collection: Abteilung Molekulare Neurobiologie     Display Documents

ID: 545959.0, MPI für medizinische Forschung / Abteilung Molekulare Neurobiologie
Tetracycline−controlled transgene activation using the ROSA26−iM2−GFP knock−in mouse strain permits GFP monitoring of DOX−regulated transgene−expression
Translation of Title:Tetracycline−controlled transgene activation using the ROSA26−iM2−GFP knock−in mouse strain permits GFP monitoring of DOX−regulated transgene−expression
Authors:Wortge, Simone; Eshkind, Leonid; Cabezas−Wallscheid, Nina; Lakaye, Bernard; Kim, Jinhyun; Heck, Rosario; Abassi, Yasmin; Diken, Mustafa; Sprengel, Rolf; Bockamp, Ernesto
Date of Publication (YYYY-MM-DD):2010-09-03
Title of Journal:BMC Developmental Biology
Journal Abbrev.:BMC Developmental Biology
Review Status:Peer-review
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:Background: Conditional gene activation is an efficient strategy for studying gene function in genetically modified
animals. Among the presently available gene switches, the tetracycline−regulated system has attracted considerable
interest because of its unique potential for reversible and adjustable gene regulation.
Results: To investigate whether the ubiquitously expressed Gt(ROSA)26Sor locus enables uniform DOX−controlled
gene expression, we inserted the improved tetracycline−regulated transcription activator iM2 together with an iM2
dependent GFP gene into the Gt(ROSA)26Sor locus, using gene targeting to generate ROSA26−iM2−GFP (R26t1Δ)
mice. Despite the presence of ROSA26 promoter driven iM2, R26t1Δ mice showed very sparse DOX−activated
expression of different iM2−responsive reporter genes in the brain, mosaic expression in peripheral tissues and
more prominent expression in erythroid, myeloid and lymphoid lineages, in hematopoietic stem and progenitor
cells and in olfactory neurons.
Conclusions: The finding that gene regulation by the DOX−activated transcriptional factor iM2 in the Gt(ROSA)
26Sor locus has its limitations is of importance for future experimental strategies involving transgene activation
from the endogenous ROSA26 promoter. Furthermore, our ROSA26−iM2 knock−in mouse model (R26t1Δ) represents
a useful tool for implementing gene function in vivo especially under circumstances requiring the side−by−side
comparison of gene manipulated and wild type cells. Since the ROSA26−iM2 mouse allows mosaic gene activation
in peripheral tissues and haematopoietic cells, this model will be very useful for uncovering previously unknown or
unsuspected phenotypes.
Last Change of the Resource (YYYY-MM-DD):--
External Publication Status:published
Document Type:Article
Communicated by:Wulf Kaiser
Affiliations:MPI für medizinische Forschung/Abteilung Molekulare Neurobiologie
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