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          Institute: MPI für medizinische Forschung     Collection: Abteilung Biomedizinische Optik     Display Documents



ID: 545973.0, MPI für medizinische Forschung / Abteilung Biomedizinische Optik
Reconstruction of firing rate changes across neuronal populations by temporally deconvolved Ca2+ imaging
Translation of Title:Reconstruction of firing rate changes across neuronal populations by temporally deconvolved Ca<sup>2+</sup> imaging
Authors:Yaksi, Emre; Friedrich, Rainer W.
Language:English
Date of Publication (YYYY-MM-DD):2006-04-20
Title of Journal:Nature Methods
Journal Abbrev.:Nature Methods
Volume:3
Issue / Number:5
Start Page:377
End Page:383
Review Status:Peer-review
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:Methods to record action potential (AP) firing in many individual neurons are essential to unravel the function of complex neuronal circuits in the brain. A promising approach is bolus loading of Ca2+ indicators combined with multiphoton microscopy. Currently, however, this technique lacks cell−type specificity, has low temporal resolution and cannot resolve complex temporal firing patterns. Here we present simple solutions to these problems. We identified neuron types by colocalizing Ca2+ signals of a red−fluorescing indicator with genetically encoded markers. We reconstructed firing rate changes from Ca2+ signals by temporal deconvolution. This technique is efficient, dramatically enhances temporal resolution, facilitates data interpretation and permits analysis of odor−response patterns across thousands of neurons in the zebrafish olfactory bulb. Hence, temporally deconvolved Ca2+ imaging (TDCa imaging) resolves limitations of current optical recording techniques and is likely to be widely applicable because of its simplicity, robustness and generic principle.
Last Change of the Resource (YYYY-MM-DD):--
External Publication Status:published
Document Type:Article
Communicated by:Wulf Kaiser
Affiliations:MPI für medizinische Forschung/Abteilung Biomedizinische Optik
Identifiers:LOCALID:6568
URI:http://www.nature.com/nmeth/journal/v3/n5/pdf/nmet...
URI:http://www.nature.com/nmeth/journal/v3/n5/full/nme...
URI:http://www.nature.com/nmeth/journal/v3/n5/index.ht...
DOI:10.1038/nmeth874
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