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          Institute: MPI für medizinische Forschung     Collection: Abteilung Biomedizinische Optik     Display Documents



ID: 545981.0, MPI für medizinische Forschung / Abteilung Biomedizinische Optik
Two−Photon Imaging Reveals Somatodendritic Chloride Gradient in Retinal ON−Type Bipolar Cells Expressing the Biosensor Clomeleon
Translation of Title:Two−Photon Imaging Reveals Somatodendritic Chloride Gradient in Retinal ON−Type Bipolar Cells Expressing the Biosensor Clomeleon
Authors:Dübel, Jens; Haverkamp, Silke; Schleich, Wolfram; Feng, Guoping; Augustine, George J.; Kuner, Thomas; Euler, Thomas
Language:English
Date of Publication (YYYY-MM-DD):2006-01-05
Title of Journal:Neuron
Journal Abbrev.:Neuron
Volume:49
Issue / Number:1
Start Page:81
End Page:94
Review Status:Peer-review
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:A somatodendritic gradient of Cl− concentration ([Cl−]i) has been postulated to generate GABA−evoked responses of different polarity in retinal bipolar cells, hyperpolarizing in OFF cells with low dendritic [Cl−]i, and depolarizing in ON cells with high dendritic [Cl−]i. As glutamate released by the photoreceptors depolarizes OFF cells and hyperpolarizes ON cells, the bipolars' antagonistic receptive field (RF) could be computed by simply integrating glutamatergic inputs from the RF center and GABAergic inputs from horizontal cells in the RF surround. Using ratiometric two−photon imaging of Clomeleon, a Cl− indicator transgenically expressed in ON bipolar cells, we found that dendritic [Cl−]i exceeds somatic [Cl−]i by up to 20 mM and that GABA application can lead to Cl− efflux (depolarization) in these dendrites. Blockers of Cl− transporters reduced the somatodendritic [Cl−]i gradient. Hence, our results support the idea that ON bipolar cells employ a somatodendritic [Cl−]i gradient to invert GABAergic horizontal cell input.
Free Keywords:SIGNALING; MOLNEURO
Last Change of the Resource (YYYY-MM-DD):--
External Publication Status:published
Document Type:Article
Communicated by:Wulf Kaiser
Affiliations:MPI für medizinische Forschung/Abteilung Biomedizinische Optik
MPI für medizinische Forschung/Abteilung Zellphysiologie
Identifiers:LOCALID:6523
URI:http://www.sciencedirect.com/science?_ob=ArticleUR...
URI:http://www.sciencedirect.com/science?_ob=ArticleUR...
URI:http://www.sciencedirect.com/science?_ob=ArticleUR...
DOI:10.1016/j.neuron.2005.10.035
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