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          Institute: MPI für medizinische Forschung     Collection: Abteilung Biomedizinische Optik     Display Documents



ID: 546035.0, MPI für medizinische Forschung / Abteilung Biomedizinische Optik
Time lapse in vivo visualization of developmental stabilization of synaptic receptors at Neuromuscular Junctions
Translation of Title:Time lapse in vivo visualization of developmental stabilization of synaptic receptors at Neuromuscular Junctions
Authors:Yampolsky, Pessah; Pacifici, Pier Giorgio; Lomb, Lukas; Giese, Günter; Rudolf, Ruediger; Roeder, Ira V.; Witzemann, Veit
Language:English
Date of Publication (YYYY-MM-DD):2010-09-02
Title of Journal:Journal of Biological Chemistry
Journal Abbrev.:J. Biol. Chem.
Volume:285
Issue / Number:45
Start Page:34589
End Page:34596
Review Status:Peer-review
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:The lifetime of nicotinic acetylcholine receptors (AChRs) in neuromuscular junctions (NMJs) is increased from <1 day to >1 week during early postnatal development. However, the exact timing of AChR stabilization is not known and its correlation to the concurrent embryonic to adult AChR channel conversion, NMJ remodeling, and neuromuscular diseases is unclear. Using a novel time−lapse in vivo imaging technology we show that replacement of the entire receptor population of an individual NMJ occurs endplate−specific within hours. This makes it possible to follow directly in live animals changing stabilities of endplate receptors. In three different, genetically modified mouse models we demonstrate that the metabolic half−life values of synaptic AChRs increase from a few hours to several days after postnatal day 6. Developmental stabilization is independent of receptor subtype and apparently regulated by an intrinsic muscle−specific maturation program. Myosin Va, a F−actin−dependent motor protein, is also accumulated synaptically during postnatal development and thus could mediate the stabilization of endplate AChR.
Last Change of the Resource (YYYY-MM-DD):--
External Publication Status:published
Document Type:Article
Communicated by:Wulf Kaiser
Affiliations:MPI für medizinische Forschung/Abteilung Biomedizinische Optik
MPI für medizinische Forschung/Abteilung Biomolekulare Mechanismen
MPI für medizinische Forschung/Arbeitsgruppe Witzemann / Koenen
Identifiers:LOCALID:7605
URI:http%3A%2F%2Fwww.jbc.org%2Fcontent%2Fearly%2F2010%...
URI:http%3A%2F%2Fwww.jbc.org%2Fcontent%2Fearly%2F2010%...
DOI:10.1074%2Fjbc.M110.168880
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