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          Institute: MPI für molekulare Zellbiologie und Genetik     Collection: Publikationen MPI-CBG 2010-arch     Display Documents



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ID: 546553.0, MPI für molekulare Zellbiologie und Genetik / Publikationen MPI-CBG 2010-arch
Correlative time-lapse imaging and electron microscopy to study abscission in HeLa cells
Authors:Guizetti, Julien; Mäntler, Jana; Müller-Reichert, Thomas; Gerlich, Daniel W
Place of Publication:Amsterdam, Netherlands
Publisher:Elsevier
Date of Publication (YYYY-MM-DD):2010
Title of Book:Electron microscopy of model systems
Start Page:591
End Page:601
Sequence Number of Inbook:24
Full Name of Book-Editor(s):Müller-Reichert, Thomas
Copyright:not available
Review Status:not specified
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:HeLa cells are widely used as a model system to study cell division. The last step of cell division, abscission, occurs at an about 1 μm wide intercellular bridge that connects the post-mitotic sister cells. Abscission often occurs long after ingression of the cleavage furrow, and no efficient methods to synchronize cells to this stage are available. Here, we have developed a correlative fluorescence time-lapse imaging and electron microscopic approach using Aclar sheets with engraved grid patterns. This grid pattern, leaving a negative imprint on thin-layer embedded samples, allows identification of cells selected from the time-lapse imaging for serial-section electron microscopy. This method facilitates the ultrastructural analysis of specific stages of abscission.
External Publication Status:published
Document Type:InBook
Communicated by:nn
Affiliations:MPI für molekulare Zellbiologie und Genetik
Identifiers:LOCALID:4425
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